Detailed information of TA system TA046998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 412865..413424
• Replicon: chromosome
• Accession: NZ_CP007763
• Organism: Brucella melitensis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q8YH01
• Locus tag: DK63_RS07655
• Protein ID: WP_004683741.1
• Coordinates: 413179..413424 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: DK63_RS07650
• Protein ID: WP_004683740.1
• Coordinates: 412865..413179 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK63_RS07600	408570..408773	-	204	WP_004683737.1	hypothetical protein	-
DK63_RS07605	408776..408991	-	216	WP_005968947.1	hypothetical protein	-
DK63_RS07610	408988..409191	-	204	WP_002964088.1	hypothetical protein	-
DK63_RS07615	409241..409945	+	705	WP_005968944.1	hypothetical protein	-
DK63_RS07620	409942..410172	+	231	WP_002964091.1	hypothetical protein	-
DK63_RS07625	410169..410444	+	276	WP_002964092.1	hypothetical protein	-
DK63_RS07630	410467..411054	+	588	WP_004683738.1	hypothetical protein	-
DK63_RS07635	411289..411999	+	711	WP_004683739.1	porin family protein	-
DK63_RS07640	412347..412517	-	171	WP_004686810.1	YegP family protein	-
DK63_RS07650	412865..413179	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
DK63_RS07655	413179..413424	-	246	WP_004683741.1	BrnT family toxin	Toxin
DK63_RS07660	413557..414195	+	639	Protein_403	tRNA-guanine transglycosylase	-
DK63_RS17620	414265..415022	+	758	WP_196219767.1	IS5 family transposase	-
DK63_RS07675	415023..415796	+	774	WP_002967634.1	hypothetical protein	-
DK63_RS07680	416127..417578	+	1452	WP_004685623.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	406798..420894	14096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9196.83 Da
• Isoelectric Point: 10.3158

>T46998 WP_004683741.1 NZ_CP007763:c413424-413179 [Brucella melitensis bv. 1 str. 16M]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVFFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T46998 NZ_CP007763:c413424-413179 [Brucella melitensis bv. 1 str. 16M]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCTTTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT46998 WP_004683740.1 NZ_CP007763:c413179-412865 [Brucella melitensis bv. 1 str. 16M]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46998 NZ_CP007763:c413179-412865 [Brucella melitensis bv. 1 str. 16M]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: Q8YH01

Antitoxin

• AlphaFold DB: C0RIV1