Detailed information of TA system TA046997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 457283..457842
• Replicon: chromosome
• Accession: NZ_CP007760
• Organism: Brucella melitensis bv. 3 str. Ether

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: DK62_RS02155
• Protein ID: WP_002966807.1
• Coordinates: 457283..457528 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: DK62_RS02160
• Protein ID: WP_004683740.1
• Coordinates: 457528..457842 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK62_RS02135	453128..454579	-	1452	WP_004685623.1	hypothetical protein	-
DK62_RS02140	454910..455683	-	774	WP_002967634.1	hypothetical protein	-
DK62_RS17625	455684..456442	-	759	Protein_427	IS5 family transposase	-
DK62_RS02150	456512..457150	-	639	Protein_428	tRNA-guanine transglycosylase	-
DK62_RS02155	457283..457528	+	246	WP_002966807.1	BrnT family toxin	Toxin
DK62_RS02160	457528..457842	+	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
DK62_RS02170	458190..458360	+	171	WP_004685622.1	YegP family protein	-
DK62_RS02175	458708..459418	-	711	WP_004685621.1	porin family protein	-
DK62_RS02180	459764..460240	-	477	WP_002966806.1	hypothetical protein	-
DK62_RS02185	460263..460538	-	276	WP_002964092.1	hypothetical protein	-
DK62_RS02190	460535..460765	-	231	WP_002964091.1	hypothetical protein	-
DK62_RS02195	460762..461466	-	705	WP_005968944.1	hypothetical protein	-
DK62_RS02200	461516..461719	+	204	WP_002964088.1	hypothetical protein	-
DK62_RS02205	461716..461931	+	216	WP_005968947.1	hypothetical protein	-
DK62_RS02210	461934..462137	+	204	WP_004683737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	450014..463909	13895
	-	flank	IS/Tn	-	-	456074..456442	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T46997 WP_002966807.1 NZ_CP007760:457283-457528 [Brucella melitensis bv. 3 str. Ether]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T46997 NZ_CP007760:457283-457528 [Brucella melitensis bv. 3 str. Ether]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT46997 WP_004683740.1 NZ_CP007760:457528-457842 [Brucella melitensis bv. 3 str. Ether]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46997 NZ_CP007760:457528-457842 [Brucella melitensis bv. 3 str. Ether]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1