Detailed information of TA system TA046996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1055806..1056365
• Replicon: chromosome
• Accession: NZ_CP007758
• Organism: Brucella canis strain RM6/66

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DK60_RS05085
• Protein ID: WP_004688321.1
• Coordinates: 1056120..1056365 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: DK60_RS05080
• Protein ID: WP_002964097.1
• Coordinates: 1055806..1056120 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK60_RS05035	1051495..1051698	-	204	WP_004690812.1	hypothetical protein	-
DK60_RS16255	1051701..1051916	-	216	WP_041594673.1	hypothetical protein	-
DK60_RS05040	1051913..1052116	-	204	WP_002964088.1	hypothetical protein	-
DK60_RS05045	1052166..1052885	+	720	WP_006072696.1	hypothetical protein	-
DK60_RS05050	1052882..1053112	+	231	WP_002964091.1	hypothetical protein	-
DK60_RS05055	1053109..1053384	+	276	WP_002964092.1	hypothetical protein	-
DK60_RS05060	1053407..1053994	+	588	WP_004683738.1	hypothetical protein	-
DK60_RS05065	1054229..1054940	+	712	Protein_998	porin family protein	-
DK60_RS05070	1055288..1055458	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DK60_RS05080	1055806..1056120	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
DK60_RS05085	1056120..1056365	-	246	WP_004688321.1	BrnT family toxin	Toxin
DK60_RS05090	1056498..1057136	+	639	Protein_1002	tRNA-guanine transglycosylase	-
DK60_RS17325	1057206..1057963	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
DK60_RS05105	1057964..1058737	+	774	WP_006132649.1	hypothetical protein	-
DK60_RS05110	1058872..1060518	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	1049723..1063834	14111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46996 WP_004688321.1 NZ_CP007758:c1056365-1056120 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46996 NZ_CP007758:c1056365-1056120 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46996 WP_002964097.1 NZ_CP007758:c1056120-1055806 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46996 NZ_CP007758:c1056120-1055806 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA