46989

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2522397..2523436 Replicon chromosome
Accession NZ_CP007748
Organism Burkholderia cepacia ATCC 25416

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A806V135
Locus tag DM41_RS36230 Protein ID WP_027791437.1
Coordinates 2522861..2523436 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A806V2D4
Locus tag DM41_RS36225 Protein ID WP_027791436.1
Coordinates 2522397..2522864 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DM41_RS36200 2517857..2519320 + 1464 WP_027791432.1 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase -
DM41_RS36205 2519370..2520218 + 849 WP_027791433.1 3,4-dihydroxyphenylacetate 2,3-dioxygenase -
DM41_RS36210 2520252..2520644 + 393 WP_027791434.1 5-carboxymethyl-2-hydroxymuconate Delta-isomerase -
DM41_RS36215 2520665..2521468 + 804 WP_021164056.1 2-oxo-hepta-3-ene-1,7-dioic acid hydratase -
DM41_RS36220 2521481..2522287 + 807 WP_027791435.1 4-hydroxy-2-oxoheptanedioate aldolase -
DM41_RS36225 2522397..2522864 + 468 WP_027791436.1 helix-turn-helix domain-containing protein Antitoxin
DM41_RS36230 2522861..2523436 + 576 WP_027791437.1 PIN domain-containing protein Toxin
DM41_RS36235 2523492..2524271 - 780 WP_027791438.1 helix-turn-helix transcriptional regulator -
DM41_RS36240 2524367..2524618 + 252 WP_027791439.1 hypothetical protein -
DM41_RS36245 2524665..2525630 + 966 WP_027791440.1 zinc-binding alcohol dehydrogenase family protein -
DM41_RS36250 2525801..2526892 - 1092 WP_034196044.1 porin -
DM41_RS36255 2527052..2528119 - 1068 WP_027791442.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21502.82 Da        Isoelectric Point: 5.6556

>T46989 WP_027791437.1 NZ_CP007748:2522861-2523436 [Burkholderia cepacia ATCC 25416]
MTSSPFTAVYDACVLYPAPLRDFLMWLALSGCFRARWSVDIHDEWKRNLLRNRADLTAAQLDRTASLMDRAIPDALVTGY
EALIGGLTLPDANDRHVLAAAIRCNASVIVTFNQKDFPEEVLSIYGVEAQHPDVFVDNLFDLEPAAVVAAARRQRETLKH
PPMIVERYLEILQRLGMVQTCKALEAYRTII

Download         Length: 576 bp

>T46989 NZ_CP007748:2522861-2523436 [Burkholderia cepacia ATCC 25416]
ATGACGAGTTCGCCATTCACGGCGGTCTACGATGCGTGCGTGCTTTATCCCGCACCGCTGCGTGATTTCCTGATGTGGCT
GGCGCTGAGCGGATGTTTTCGGGCTCGCTGGAGTGTGGATATTCATGACGAATGGAAACGGAATCTGCTGCGTAACCGGG
CCGATCTGACCGCGGCGCAACTCGATCGCACGGCAAGCCTGATGGATAGGGCGATACCCGATGCGCTCGTTACCGGTTAC
GAGGCGCTGATTGGTGGGCTGACGCTGCCGGACGCGAACGATCGACATGTGCTCGCTGCCGCGATCCGCTGCAACGCCAG
CGTGATCGTTACGTTCAATCAGAAGGATTTCCCCGAAGAAGTGTTGTCGATCTACGGTGTCGAAGCACAGCACCCTGACG
TGTTCGTCGACAATCTCTTCGATCTCGAACCGGCCGCAGTGGTCGCCGCCGCTCGGCGGCAGCGCGAAACACTCAAGCAT
CCACCGATGATTGTGGAGCGCTATCTGGAAATCTTGCAGCGTCTGGGGATGGTTCAGACCTGCAAGGCACTCGAAGCCTA
TCGGACGATTATTTGA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17497.87 Da        Isoelectric Point: 6.2956

>AT46989 WP_027791436.1 NZ_CP007748:2522397-2522864 [Burkholderia cepacia ATCC 25416]
MPHTTMSETILPVEREMRAAVEGQRALAAYLATRTEIQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPHHKTGKHRRVRFSDLMRYKAERDQASADAMEELSRQAQEHGMGYE

Download         Length: 468 bp

>AT46989 NZ_CP007748:2522397-2522864 [Burkholderia cepacia ATCC 25416]
ATGCCTCACACCACGATGTCCGAAACGATCTTGCCTGTCGAGCGCGAGATGCGGGCGGCTGTCGAAGGGCAGCGTGCGCT
TGCGGCATACCTGGCAACGCGAACGGAAATACAGCGCATCCAGATCTTCGACGACGAAAATCGCGCGCATCAGGTCGAAC
TTCCGACCTCCGCGCTGCGTCTGCTGGTCGATATCCTCGCCGAGTTGGCCGACGGCAATGCGGTCAAGGTCGTGCCGGTT
CATGCGGAACTGACGACACAGGAAGCGGCCGACCTGTTGAACGTTTCTCGTCCGCACCTGATCAAGCTGCTCGAATCGAA
TGCGCTTCCGCACCACAAGACCGGCAAGCATCGCCGTGTGCGATTTTCCGACCTGATGCGTTACAAGGCGGAACGCGATC
AGGCAAGCGCGGACGCGATGGAGGAGTTGAGCCGGCAGGCGCAAGAGCACGGGATGGGGTACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A806V135

Antitoxin

Source ID Structure
AlphaFold DB A0A806V2D4

References