Detailed information of TA system TA046988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2318511..2319088
• Replicon: chromosome
• Accession: NZ_CP007748
• Organism: Burkholderia cepacia ATCC 25416

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2G063
• Locus tag: DM41_RS35365
• Protein ID: WP_021160897.1
• Coordinates: 2318511..2318876 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: DM41_RS35370
• Protein ID: WP_021160898.1
• Coordinates: 2318870..2319088 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM41_RS35345	2314290..2314844	+	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-
DM41_RS41075	2315166..2315780	-	615	WP_155253146.1	hypothetical protein	-
DM41_RS35355	2315885..2317483	-	1599	WP_027791288.1	alkyl hydroperoxide reductase subunit F	-
DM41_RS35360	2317609..2318172	-	564	WP_006488685.1	peroxiredoxin	-
DM41_RS35365	2318511..2318876	-	366	WP_021160897.1	type II toxin-antitoxin system VapC family toxin	Toxin
DM41_RS35370	2318870..2319088	-	219	WP_021160898.1	hypothetical protein	Antitoxin
DM41_RS35375	2319179..2320075	-	897	WP_027791289.1	LysR family transcriptional regulator	-
DM41_RS35380	2320253..2321419	+	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
DM41_RS35385	2321474..2321998	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
DM41_RS35390	2321991..2323256	+	1266	WP_027791291.1	Zn-dependent hydrolase	-
DM41_RS35395	2323344..2323826	-	483	WP_027791292.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13448.50 Da
• Isoelectric Point: 8.4389

>T46988 WP_021160897.1 NZ_CP007748:c2318876-2318511 [Burkholderia cepacia ATCC 25416]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T46988 NZ_CP007748:c2318876-2318511 [Burkholderia cepacia ATCC 25416]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT46988 WP_021160898.1 NZ_CP007748:c2319088-2318870 [Burkholderia cepacia ATCC 25416]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT46988 NZ_CP007748:c2319088-2318870 [Burkholderia cepacia ATCC 25416]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: U2G063

Antitoxin

• AlphaFold DB: U2HEK9