Detailed information of TA system TA046987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1928101..1928742
• Replicon: chromosome
• Accession: NZ_CP007748
• Organism: Burkholderia cepacia ATCC 25416

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DM41_RS33530
• Protein ID: WP_027790991.1
• Coordinates: 1928404..1928742 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: DM41_RS33525
• Protein ID: WP_021157290.1
• Coordinates: 1928101..1928400 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM41_RS33500	1923215..1924486	+	1272	WP_027790987.1	Rieske 2Fe-2S domain-containing protein	-
DM41_RS33505	1924504..1924989	+	486	WP_034196026.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
DM41_RS33510	1925010..1925336	+	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
DM41_RS33515	1925326..1926546	+	1221	WP_027790989.1	FAD-dependent oxidoreductase	-
DM41_RS33520	1926758..1928044	+	1287	WP_027790990.1	DUF445 domain-containing protein	-
DM41_RS33525	1928101..1928400	-	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
DM41_RS33530	1928404..1928742	-	339	WP_027790991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DM41_RS33540	1929082..1930722	+	1641	WP_027790992.1	acetolactate synthase large subunit	-
DM41_RS33545	1930752..1932185	+	1434	WP_027790993.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12591.43 Da
• Isoelectric Point: 9.9308

>T46987 WP_027790991.1 NZ_CP007748:c1928742-1928404 [Burkholderia cepacia ATCC 25416]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLGGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T46987 NZ_CP007748:c1928742-1928404 [Burkholderia cepacia ATCC 25416]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGCGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCGGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT46987 WP_021157290.1 NZ_CP007748:c1928400-1928101 [Burkholderia cepacia ATCC 25416]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT46987 NZ_CP007748:c1928400-1928101 [Burkholderia cepacia ATCC 25416]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCATGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3