Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4088089..4088729 Replicon chromosome
Accession NZ_CP007744
Organism Pectobacterium atrosepticum strain JG10-08

Toxin (Protein)


Gene name vagD Uniprot ID Q6D0W3
Locus tag EV46_RS17905 Protein ID WP_011095186.1
Coordinates 4088089..4088505 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6D0W2
Locus tag EV46_RS17910 Protein ID WP_011095187.1
Coordinates 4088502..4088729 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EV46_RS17880 4083852..4085183 + 1332 WP_039293538.1 type I-F CRISPR-associated protein Csy1 -
EV46_RS17885 4085180..4086112 + 933 WP_011095182.1 type I-F CRISPR-associated protein Csy2 -
EV46_RS17890 4086126..4087139 + 1014 WP_011095183.1 type I-F CRISPR-associated protein Csy3 -
EV46_RS17895 4087149..4087703 + 555 WP_039293542.1 type I-F CRISPR-associated endoribonuclease Cas6/Csy4 -
EV46_RS17905 4088089..4088505 - 417 WP_011095186.1 type II toxin-antitoxin system VapC family toxin Toxin
EV46_RS17910 4088502..4088729 - 228 WP_011095187.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EV46_RS17915 4089627..4090046 - 420 WP_011095188.1 secondary thiamine-phosphate synthase enzyme YjbQ -
EV46_RS17920 4090064..4092898 - 2835 WP_039293545.1 excinuclease ABC subunit UvrA -
EV46_RS17925 4093144..4093707 + 564 WP_011095190.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15299.98 Da        Isoelectric Point: 8.7720

>T46979 WP_011095186.1 NZ_CP007744:c4088505-4088089 [Pectobacterium atrosepticum]
VSRLFMLDTNICSFIMREHPSAVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMLLVDAFCRRLDAILPWDSA
AVDATTKIKIALAAAVTPIGPNDTAIAGHAISAKAILVTNNLREFERVPELILEDWVK

Download         Length: 417 bp

>T46979 NZ_CP007744:c4088505-4088089 [Pectobacterium atrosepticum]
GTGAGCCGTTTGTTCATGCTTGATACTAATATCTGTTCTTTCATTATGCGTGAACACCCGTCTGCGGTGATTGAAAAGTT
GCAGCAATGCACACTGCGACGAGATCGAATCGTGGTATCAGCTATCACGTACGCTGAAATGCGTTTTGGGGCGGTTGGCA
AAAAAGCTTCGCCGCGCCATATGCTGCTGGTTGATGCTTTCTGCCGCCGACTTGATGCGATATTACCTTGGGATAGCGCA
GCGGTAGACGCCACCACAAAAATTAAAATTGCACTAGCTGCAGCTGTCACACCTATTGGTCCAAACGATACGGCCATCGC
TGGTCATGCTATCAGCGCTAAGGCCATACTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGAACTGATACTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8894.05 Da        Isoelectric Point: 4.5216

>AT46979 WP_011095187.1 NZ_CP007744:c4088729-4088502 [Pectobacterium atrosepticum]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEERADADFMTERQDVVEEGRFEL

Download         Length: 228 bp

>AT46979 NZ_CP007744:c4088729-4088502 [Pectobacterium atrosepticum]
ATGCGTATTGTCTCAGTATTTAAAAATGGTAATAACCGTGCTATTCGCTTGCCGCGTGATATGGACTTTGAAGGCGTCAA
TGAACTGGAGATCGTGCGTGAAGGGGATACCATCATGTTGCGCCCAGTCAAACCCAATTGGCATTCTTTTCTGCAAGAAG
AGCGTGCTGACGCAGACTTTATGACAGAACGTCAGGATGTAGTCGAAGAGGGGAGATTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6D0W3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J5XN10

References