Detailed information of TA system TA046970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 403042..403601
• Replicon: chromosome
• Accession: NZ_CP007743
• Organism: Brucella pinnipedialis strain 6/566

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DK65_RS07540
• Protein ID: WP_004688321.1
• Coordinates: 403042..403287 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D0RHG9
• Locus tag: DK65_RS07545
• Protein ID: WP_006161014.1
• Coordinates: 403287..403601 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK65_RS07515	398889..400535	-	1647	WP_004689673.1	hypothetical protein	-
DK65_RS07520	400670..401443	-	774	WP_002967634.1	hypothetical protein	-
DK65_RS07535	402271..402909	-	639	Protein_379	tRNA-guanine transglycosylase	-
DK65_RS07540	403042..403287	+	246	WP_004688321.1	BrnT family toxin	Toxin
DK65_RS07545	403287..403601	+	315	WP_006161014.1	BrnA antitoxin family protein	Antitoxin
DK65_RS07550	403949..404119	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
DK65_RS16680	404467..405176	-	710	Protein_383	porin family protein	-
DK65_RS07565	405411..405998	-	588	WP_004683738.1	hypothetical protein	-
DK65_RS07570	406021..406296	-	276	WP_002964092.1	hypothetical protein	-
DK65_RS07575	406293..406523	-	231	WP_002964091.1	hypothetical protein	-
DK65_RS07580	406520..407239	-	720	WP_006072696.1	hypothetical protein	-
DK65_RS07585	407289..407492	+	204	WP_002964088.1	hypothetical protein	-
DK65_RS07590	407489..407704	+	216	WP_002971459.1	hypothetical protein	-
DK65_RS07595	407707..407910	+	204	WP_002964087.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	395775..409681	13906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46970 WP_004688321.1 NZ_CP007743:403042-403287 [Brucella pinnipedialis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46970 NZ_CP007743:403042-403287 [Brucella pinnipedialis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11668.45 Da
• Isoelectric Point: 10.0945

>AT46970 WP_006161014.1 NZ_CP007743:403287-403601 [Brucella pinnipedialis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGWPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46970 NZ_CP007743:403287-403601 [Brucella pinnipedialis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCTGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Antitoxin

• AlphaFold DB: D0RHG9