Detailed information of TA system TA046969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 238939..239575
• Replicon: chromosome
• Accession: NZ_CP007739
• Organism: Bacillus methanolicus MGA3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3E3S0
• Locus tag: BMMGA3_RS01305
• Protein ID: WP_003348433.1
• Coordinates: 239225..239575 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: I3E3S1
• Locus tag: BMMGA3_RS01300
• Protein ID: WP_003348435.1
• Coordinates: 238939..239220 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMMGA3_RS01275	234771..235370	-	600	WP_003348445.1	rhomboid family intramembrane serine protease	-
BMMGA3_RS01280	235489..235845	+	357	WP_003348443.1	holo-ACP synthase	-
BMMGA3_RS01285	236047..237060	+	1014	WP_034669513.1	outer membrane lipoprotein carrier protein LolA	-
BMMGA3_RS01290	237338..238501	+	1164	WP_003348440.1	alanine racemase	-
BMMGA3_RS01300	238939..239220	+	282	WP_003348435.1	hypothetical protein	Antitoxin
BMMGA3_RS01305	239225..239575	+	351	WP_003348433.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMMGA3_RS01310	239914..240735	+	822	WP_003348431.1	STAS domain-containing protein	-
BMMGA3_RS01315	240732..241088	+	357	WP_003348429.1	STAS domain-containing protein	-
BMMGA3_RS01320	241093..241494	+	402	WP_003348427.1	anti-sigma regulatory factor	-
BMMGA3_RS01325	241505..242515	+	1011	WP_003348425.1	PP2C family protein-serine/threonine phosphatase	-
BMMGA3_RS01330	242572..242901	+	330	WP_003348423.1	anti-sigma factor antagonist	-
BMMGA3_RS01335	242901..243374	+	474	WP_003348421.1	anti-sigma B factor RsbW	-
BMMGA3_RS01340	243352..244146	+	795	WP_003348419.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13022.03 Da
• Isoelectric Point: 4.8998

>T46969 WP_003348433.1 NZ_CP007739:239225-239575 [Bacillus methanolicus MGA3]
MIVKRGDVYFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T46969 NZ_CP007739:239225-239575 [Bacillus methanolicus MGA3]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCGGACCTATCCCCAGTTGTTGGGTCAGAACAAGGCGGAACCCGTCCTGT
ACTAGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTTATCGTAGCAGCGATCACTGCCCAAATCCAAAAAG
CAAAGCTGCCCACACACGTTGAAATTGATGCGAAACGTTACGGTTTTGAACGAGACTCTGTCATCCTATTGGAACAAATT
CGTACAATTGATAAACAGCGATTAACCGATAAGATTACTCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCCT
TCAAATCAGTTTAGGTCTCATAGAGTTCTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10758.24 Da
• Isoelectric Point: 7.2028

>AT46969 WP_003348435.1 NZ_CP007739:238939-239220 [Bacillus methanolicus MGA3]
VSESSVTTEILVKLPQHLLNELDGFVKQENVNRSELIYQATKAYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT46969 NZ_CP007739:238939-239220 [Bacillus methanolicus MGA3]
GTGTCTGAGTCCAGTGTAACAACGGAGATCTTGGTAAAATTACCGCAACATCTTTTAAACGAATTAGATGGTTTTGTAAA
ACAAGAAAATGTAAATCGCAGCGAGCTTATTTATCAAGCAACAAAAGCGTATTTGCGTGAACGTAAAAAGAGACAAATTC
GTGAATCAATGAGGCGCGGCTATATGGAAATGGCCAAAATTAATCTGACGATTGCATCAGAAGCATTCCAAGCAGAATAT
GAGGCAGAACACACAGTTGAACGTCTAGTTAGCGGGGGATAA

Structures

Toxin

• AlphaFold DB: I3E3S0

Antitoxin

• AlphaFold DB: I3E3S1