Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12293..12818 Replicon plasmid pKPN-a41
Accession NZ_CP007735
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag KPNIH27_RS29980 Protein ID WP_004197633.1
Coordinates 12513..12818 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag KPNIH27_RS29975 Protein ID WP_006788213.1
Coordinates 12293..12511 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS29950 7748..8611 - 864 WP_042922438.1 hypothetical protein -
KPNIH27_RS29955 8893..9996 + 1104 WP_042922442.1 nucleotide-binding protein -
KPNIH27_RS29960 10026..11156 + 1131 WP_042922444.1 DUF4238 domain-containing protein -
KPNIH27_RS29965 11156..11437 + 282 WP_042922447.1 hypothetical protein -
KPNIH27_RS29970 11429..11767 - 339 WP_048994308.1 hypothetical protein -
KPNIH27_RS29975 12293..12511 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KPNIH27_RS29980 12513..12818 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
KPNIH27_RS29985 12976..13659 + 684 WP_023280892.1 hypothetical protein -
KPNIH27_RS29990 13662..14438 + 777 WP_049245386.1 site-specific integrase -
KPNIH27_RS29995 14496..14753 - 258 WP_000764642.1 hypothetical protein -
KPNIH27_RS34920 14882..14986 - 105 WP_032409716.1 hypothetical protein -
KPNIH27_RS30000 15521..16387 + 867 WP_004118283.1 replication initiation protein -
KPNIH27_RS30005 16744..17013 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..89770 89770


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T46966 WP_004197633.1 NZ_CP007735:12513-12818 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T46966 NZ_CP007735:12513-12818 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT46966 WP_006788213.1 NZ_CP007735:12293-12511 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT46966 NZ_CP007735:12293-12511 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References