Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 91032..91768 Replicon plasmid pKPN-262
Accession NZ_CP007734
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag KPNIH27_RS28690 Protein ID WP_004098919.1
Coordinates 91286..91768 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A455TK27
Locus tag KPNIH27_RS28685 Protein ID WP_020804426.1
Coordinates 91032..91298 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS28640 87214..87648 + 435 WP_004118347.1 hypothetical protein -
KPNIH27_RS28645 88027..88125 - 99 WP_004118688.1 type II toxin-antitoxin system RelE/ParE family toxin -
KPNIH27_RS28650 88112..88291 - 180 WP_004118349.1 type II toxin-antitoxin system ParD family antitoxin -
KPNIH27_RS28655 88605..88868 + 264 WP_004118691.1 hypothetical protein -
KPNIH27_RS28660 88865..89431 + 567 WP_042922197.1 hypothetical protein -
KPNIH27_RS28665 89462..89956 + 495 WP_016528789.1 hypothetical protein -
KPNIH27_RS28670 90017..90220 + 204 WP_004150739.1 hemolysin expression modulator Hha -
KPNIH27_RS28675 90269..90526 + 258 WP_004098928.1 hypothetical protein -
KPNIH27_RS28680 90602..90856 + 255 WP_032425571.1 hypothetical protein -
KPNIH27_RS28685 91032..91298 + 267 WP_020804426.1 DUF1778 domain-containing protein Antitoxin
KPNIH27_RS28690 91286..91768 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
KPNIH27_RS28695 91969..93372 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KPNIH27_RS28700 93401..94033 - 633 WP_001567369.1 hypothetical protein -
KPNIH27_RS28705 94210..95742 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkB / mrkC / mrkD / mrkF / mrkJ 1..338850 338850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T46965 WP_004098919.1 NZ_CP007734:91286-91768 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T46965 NZ_CP007734:91286-91768 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGTGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10124.47 Da        Isoelectric Point: 4.3108

>AT46965 WP_020804426.1 NZ_CP007734:91032-91298 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV

Download         Length: 267 bp

>AT46965 NZ_CP007734:91032-91298 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCCCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATGAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A455TK27

References