Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58015..58540 Replicon plasmid pKPN-262
Accession NZ_CP007734
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag KPNIH27_RS28500 Protein ID WP_004197633.1
Coordinates 58015..58320 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag KPNIH27_RS28505 Protein ID WP_006788213.1
Coordinates 58322..58540 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS28465 53177..54145 + 969 WP_000654811.1 IS5 family transposase -
KPNIH27_RS34890 54677..54790 + 114 WP_014343462.1 hypothetical protein -
KPNIH27_RS28475 54919..55176 + 258 WP_009310077.1 hypothetical protein -
KPNIH27_RS28480 55234..56013 - 780 WP_023287113.1 site-specific integrase -
KPNIH27_RS28485 56211..57227 - 1017 WP_009309921.1 hypothetical protein -
KPNIH27_RS28490 57261..57596 - 336 WP_009309920.1 hypothetical protein -
KPNIH27_RS34205 57646..57957 - 312 WP_023287114.1 hypothetical protein -
KPNIH27_RS28500 58015..58320 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
KPNIH27_RS28505 58322..58540 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KPNIH27_RS28510 59137..59367 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
KPNIH27_RS28515 59364..59780 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
KPNIH27_RS28520 59821..60699 - 879 WP_009309916.1 restriction endonuclease -
KPNIH27_RS28525 61361..62422 + 1062 Protein_68 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkB / mrkC / mrkD / mrkF / mrkJ 1..338850 338850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T46963 WP_004197633.1 NZ_CP007734:c58320-58015 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T46963 NZ_CP007734:c58320-58015 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT46963 WP_006788213.1 NZ_CP007734:c58540-58322 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT46963 NZ_CP007734:c58540-58322 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References