Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-YefM
Location 247235..247806 Replicon plasmid pKEC-dc3
Accession NZ_CP007732
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name parE Uniprot ID A0A2I8S8S5
Locus tag KPNIH27_RS32645 Protein ID WP_011918375.1
Coordinates 247495..247806 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1Z3MW28
Locus tag KPNIH27_RS27575 Protein ID WP_011787805.1
Coordinates 247235..247495 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS27550 242444..243073 + 630 WP_044489169.1 3'-5' exonuclease -
KPNIH27_RS27555 243525..245015 + 1491 WP_011787801.1 SulP family inorganic anion transporter -
KPNIH27_RS27560 245050..245904 + 855 WP_011787802.1 universal stress protein -
KPNIH27_RS27565 245995..246327 + 333 WP_011787803.1 TraR/DksA C4-type zinc finger protein -
KPNIH27_RS27570 246445..247065 - 621 WP_011787804.1 recombinase family protein -
KPNIH27_RS27575 247235..247495 + 261 WP_011787805.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPNIH27_RS32645 247495..247806 + 312 WP_011918375.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPNIH27_RS27580 247830..250910 + 3081 WP_011787830.1 Tn3 family transposase -
KPNIH27_RS27585 250964..251254 + 291 Protein_281 3'-5' exonuclease -
KPNIH27_RS27590 251251..251742 + 492 WP_000139717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaFOX-5 / dfrA14 / ant(2'')-Ia / qacE / sul1 / msr(E) / mph(E) / blaKPC-2 / blaSHV-5 / aac(3)-Ia - 1..268334 268334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-92)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-61)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11862.70 Da        Isoelectric Point: 8.5341

>T46962 WP_011918375.1 NZ_CP007732:247495-247806 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MPVTYHLTPDAQSDLIGIHRFTLAQWGTTQSKTYLSGLRQTIQLLAETPTLGKNRPEVRMNVFSFPYSSHVIYYIQHEHQ
FVVFGILHKSMVPLAHLAEREII

Download         Length: 312 bp

>T46962 NZ_CP007732:247495-247806 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGCCGGTAACTTATCATTTAACGCCCGATGCACAATCTGATCTGATAGGTATTCACCGCTTTACGTTAGCCCAATGGGG
AACGACTCAGTCGAAGACCTATTTATCAGGACTTAGACAAACGATTCAACTGCTGGCTGAAACACCCACCCTTGGGAAAA
ATAGACCCGAGGTACGCATGAATGTGTTTAGCTTCCCTTATTCAAGTCATGTCATCTATTACATCCAACATGAGCATCAA
TTCGTTGTATTTGGGATCTTACATAAAAGTATGGTTCCACTTGCACATCTCGCGGAGCGGGAAATTATTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9312.71 Da        Isoelectric Point: 6.4546

>AT46962 WP_011787805.1 NZ_CP007732:247235-247495 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MNTLSANEAKIHFGDLLLKAQQAPIQINKNGKPVAVVISADAYQSIETLKLHLLQSKAVKAITDIKMGNLVDGNTFFDEL
AAGQYD

Download         Length: 261 bp

>AT46962 NZ_CP007732:247235-247495 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAACACTCTTTCAGCCAATGAAGCAAAAATTCACTTTGGAGATTTGTTGCTCAAAGCTCAACAAGCTCCAATCCAAAT
TAATAAAAATGGCAAGCCTGTGGCCGTTGTTATTTCAGCTGATGCATACCAAAGTATCGAGACACTAAAATTACATTTGC
TTCAATCCAAAGCGGTGAAAGCCATAACTGACATCAAAATGGGCAATTTGGTTGATGGTAATACCTTTTTTGATGAGCTG
GCCGCAGGGCAGTATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S8S5

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MW28

References