Detailed information of TA system TA046958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4471758..4472536
• Replicon: chromosome
• Accession: NZ_CP007731
• Organism: Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: KPNIH27_RS22250
• Protein ID: WP_031591140.1
• Coordinates: 4471758..4472246 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: KPNIH27_RS22255
• Protein ID: WP_004150912.1
• Coordinates: 4472243..4472536 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH27_RS22245	4467933..4471677	+	3745	Protein_4272	hypothetical protein	-
KPNIH27_RS22250	4471758..4472246	-	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
KPNIH27_RS22255	4472243..4472536	-	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
KPNIH27_RS22260	4473181..4474557	+	1377	WP_004181304.1	cystathionine beta-synthase	-
KPNIH27_RS22265	4474568..4475716	+	1149	WP_032442510.1	PLP-dependent aspartate aminotransferase family protein	-
KPNIH27_RS22270	4475717..4476628	-	912	WP_032417598.1	LysR family transcriptional regulator	-
KPNIH27_RS22275	4476726..4477328	+	603	WP_032442511.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T46958 WP_031591140.1 NZ_CP007731:c4472246-4471758 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T46958 NZ_CP007731:c4472246-4471758 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTGTGTTGCGGCAGCGATTCTAACGTAC
TGGCGTATTACTCGCTCGCGTCCAGTGCGGTAATGACTAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTAGTAGTGCTGGGGCGTCTGGCGGTGGATCAGTCTCTACATGGACGGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGGGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTATCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT46958 WP_004150912.1 NZ_CP007731:c4472536-4472243 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT46958 NZ_CP007731:c4472536-4472243 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4