Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4397298..4397955 Replicon chromosome
Accession NZ_CP007731
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag KPNIH27_RS21895 Protein ID WP_002916310.1
Coordinates 4397298..4397708 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag KPNIH27_RS21900 Protein ID WP_002916312.1
Coordinates 4397689..4397955 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS21875 4393298..4395031 - 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -
KPNIH27_RS21880 4395037..4395750 - 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KPNIH27_RS21885 4395773..4396669 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
KPNIH27_RS21890 4396770..4397291 + 522 WP_004144730.1 flavodoxin FldB -
KPNIH27_RS21895 4397298..4397708 - 411 WP_002916310.1 protein YgfX Toxin
KPNIH27_RS21900 4397689..4397955 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
KPNIH27_RS21905 4398201..4399184 + 984 WP_002916313.1 tRNA-modifying protein YgfZ -
KPNIH27_RS21910 4399335..4399994 - 660 WP_002916317.1 hemolysin III family protein -
KPNIH27_RS21915 4400158..4400469 - 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
KPNIH27_RS21920 4400519..4401247 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
KPNIH27_RS21925 4401366..4402799 + 1434 WP_042921222.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T46957 WP_002916310.1 NZ_CP007731:c4397708-4397298 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T46957 NZ_CP007731:c4397708-4397298 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT46957 WP_002916312.1 NZ_CP007731:c4397955-4397689 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46957 NZ_CP007731:c4397955-4397689 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References