Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 524168..524684 Replicon chromosome
Accession NZ_CP007731
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name relE Uniprot ID A0A085DK79
Locus tag KPNIH27_RS02620 Protein ID WP_009309309.1
Coordinates 524400..524684 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag KPNIH27_RS02615 Protein ID WP_002886901.1
Coordinates 524168..524410 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS02600 520196..520936 + 741 WP_002886899.1 KDGP aldolase family protein -
KPNIH27_RS02605 521003..522157 + 1155 WP_023280781.1 lactonase family protein -
KPNIH27_RS02610 522180..524090 + 1911 WP_023280782.1 BglG family transcription antiterminator -
KPNIH27_RS02615 524168..524410 + 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KPNIH27_RS02620 524400..524684 + 285 WP_009309309.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPNIH27_RS02625 524688..525152 - 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
KPNIH27_RS02630 525393..527531 - 2139 WP_032442589.1 anaerobic ribonucleoside-triphosphate reductase -
KPNIH27_RS02640 527888..528631 - 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
KPNIH27_RS34385 528634..528807 - 174 WP_032412860.1 hypothetical protein -
KPNIH27_RS02650 528892..529200 + 309 WP_020079792.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11141.99 Da        Isoelectric Point: 10.3787

>T46944 WP_009309309.1 NZ_CP007731:524400-524684 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNLRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T46944 NZ_CP007731:524400-524684 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCTGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT46944 WP_002886901.1 NZ_CP007731:524168-524410 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT46944 NZ_CP007731:524168-524410 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A085DK79

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References