Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 83739..84475 Replicon plasmid pKPN-498
Accession NZ_CP007729
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH10

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPNIH10_RS27780 Protein ID WP_003026803.1
Coordinates 83739..84221 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPNIH10_RS27785 Protein ID WP_003026799.1
Coordinates 84209..84475 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH10_RS27760 79376..80338 + 963 WP_004152113.1 M48 family metalloprotease -
KPNIH10_RS31775 80426..81680 + 1255 Protein_81 IS3 family transposase -
KPNIH10_RS27775 82181..83527 - 1347 WP_020314316.1 ISNCY family transposase -
KPNIH10_RS27780 83739..84221 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPNIH10_RS27785 84209..84475 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPNIH10_RS27790 84651..84905 - 255 WP_004152108.1 hypothetical protein -
KPNIH10_RS27795 84981..85238 - 258 WP_004152107.1 hypothetical protein -
KPNIH10_RS27800 85287..85490 - 204 WP_004152106.1 hemolysin expression modulator Hha -
KPNIH10_RS27805 85524..85892 - 369 WP_004152105.1 hypothetical protein -
KPNIH10_RS27810 85936..86430 - 495 WP_004152104.1 DNA-binding protein -
KPNIH10_RS27815 86461..87036 - 576 WP_004152103.1 hypothetical protein -
KPNIH10_RS27820 87024..87293 - 270 WP_004152102.1 hypothetical protein -
KPNIH10_RS27825 87651..88001 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPNIH10_RS27830 88051..88413 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..243824 243824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T46940 WP_003026803.1 NZ_CP007729:c84221-83739 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T46940 NZ_CP007729:c84221-83739 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT46940 WP_003026799.1 NZ_CP007729:c84475-84209 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT46940 NZ_CP007729:c84475-84209 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References