Detailed information of TA system TA046919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 958860..959419
• Replicon: chromosome
• Accession: NZ_CP007720
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSSP3_RS04695
• Protein ID: WP_004688321.1
• Coordinates: 959174..959419 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSSP3_RS04690
• Protein ID: WP_002964097.1
• Coordinates: 958860..959174 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSSP3_RS04645	954550..954753	-	204	WP_004690812.1	hypothetical protein	-
BSSP3_RS04650	954756..954971	-	216	WP_002971459.1	hypothetical protein	-
BSSP3_RS04655	954968..955171	-	204	WP_002964088.1	hypothetical protein	-
BSSP3_RS04660	955221..955940	+	720	WP_006072696.1	hypothetical protein	-
BSSP3_RS04665	955937..956167	+	231	WP_002964091.1	hypothetical protein	-
BSSP3_RS04670	956164..956439	+	276	WP_002964092.1	hypothetical protein	-
BSSP3_RS04675	956462..957049	+	588	WP_004683738.1	hypothetical protein	-
BSSP3_RS04680	957284..957994	+	711	WP_004683739.1	porin family protein	-
BSSP3_RS04685	958342..958512	-	171	WP_006205664.1	DUF1508 domain-containing protein	-
BSSP3_RS04690	958860..959174	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSSP3_RS04695	959174..959419	-	246	WP_004688321.1	BrnT family toxin	Toxin
BSSP3_RS04700	959552..960190	+	639	Protein_916	tRNA-guanine transglycosylase	-
BSSP3_RS17530	960260..961017	+	758	WP_144313581.1	IS5 family transposase	-
BSSP3_RS04715	961018..961791	+	774	WP_006072701.1	hypothetical protein	-
BSSP3_RS04720	961926..963572	+	1647	WP_006072703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	953538..966888	13350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46919 WP_004688321.1 NZ_CP007720:c959419-959174 [Brucella suis bv. 2]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46919 NZ_CP007720:c959419-959174 [Brucella suis bv. 2]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46919 WP_002964097.1 NZ_CP007720:c959174-958860 [Brucella suis bv. 2]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46919 NZ_CP007720:c959174-958860 [Brucella suis bv. 2]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA