Detailed information of TA system TA046918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1940179..1940738
• Replicon: chromosome
• Accession: NZ_CP007719
• Organism: Brucella suis bv. 3 str. 686

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DK67_RS14875
• Protein ID: WP_004688321.1
• Coordinates: 1940493..1940738 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: DK67_RS14870
• Protein ID: WP_002964097.1
• Coordinates: 1940179..1940493 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK67_RS14825	1935869..1936072	-	204	WP_004690812.1	hypothetical protein	-
DK67_RS14830	1936075..1936290	-	216	WP_002971459.1	hypothetical protein	-
DK67_RS14835	1936287..1936490	-	204	WP_002964088.1	hypothetical protein	-
DK67_RS14840	1936540..1937259	+	720	WP_006072696.1	hypothetical protein	-
DK67_RS14845	1937256..1937486	+	231	WP_002964091.1	hypothetical protein	-
DK67_RS14850	1937483..1937758	+	276	WP_002964092.1	hypothetical protein	-
DK67_RS14855	1937781..1938368	+	588	WP_004683738.1	hypothetical protein	-
DK67_RS14860	1938603..1939313	+	711	WP_004683739.1	porin family protein	-
DK67_RS14865	1939661..1939831	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DK67_RS14870	1940179..1940493	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
DK67_RS14875	1940493..1940738	-	246	WP_004688321.1	BrnT family toxin	Toxin
DK67_RS14880	1940871..1941509	+	639	Protein_1831	tRNA-guanine transglycosylase	-
DK67_RS17420	1941579..1942336	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
DK67_RS14895	1942337..1943110	+	774	WP_006132649.1	hypothetical protein	-
DK67_RS14900	1943245..1944891	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	1934097..1948207	14110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46918 WP_004688321.1 NZ_CP007719:c1940738-1940493 [Brucella suis bv. 3 str. 686]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46918 NZ_CP007719:c1940738-1940493 [Brucella suis bv. 3 str. 686]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46918 WP_002964097.1 NZ_CP007719:c1940493-1940179 [Brucella suis bv. 3 str. 686]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46918 NZ_CP007719:c1940493-1940179 [Brucella suis bv. 3 str. 686]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA