Detailed information of TA system TA046917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 229543..230102
• Replicon: chromosome
• Accession: NZ_CP007717
• Organism: Brucella suis strain 513UK

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DM38_RS06675
• Protein ID: WP_004688321.1
• Coordinates: 229857..230102 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: DM38_RS06670
• Protein ID: WP_002964097.1
• Coordinates: 229543..229857 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM38_RS06625	225233..225436	-	204	WP_002964087.1	hypothetical protein	-
DM38_RS06630	225439..225654	-	216	WP_002971459.1	hypothetical protein	-
DM38_RS06635	225651..225854	-	204	WP_002964088.1	hypothetical protein	-
DM38_RS06640	225904..226623	+	720	WP_006072696.1	hypothetical protein	-
DM38_RS06645	226620..226850	+	231	WP_002964091.1	hypothetical protein	-
DM38_RS06650	226847..227122	+	276	WP_002964092.1	hypothetical protein	-
DM38_RS06655	227145..227732	+	588	WP_004683738.1	hypothetical protein	-
DM38_RS06660	227967..228677	+	711	WP_004689672.1	porin family protein	-
DM38_RS06665	229025..229195	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DM38_RS17585	229194..229427	+	234	WP_002969999.1	hypothetical protein	-
DM38_RS06670	229543..229857	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
DM38_RS06675	229857..230102	-	246	WP_004688321.1	BrnT family toxin	Toxin
DM38_RS06680	230235..230873	+	639	Protein_224	tRNA-guanine transglycosylase	-
DM38_RS17255	230943..231701	+	759	Protein_225	IS5 family transposase	-
DM38_RS06690	231702..232475	+	774	WP_002967634.1	hypothetical protein	-
DM38_RS06695	232610..234256	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	224221..237572	13351
	-	flank	IS/Tn	-	-	230943..231311	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46917 WP_004688321.1 NZ_CP007717:c230102-229857 [Brucella suis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46917 NZ_CP007717:c230102-229857 [Brucella suis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46917 WP_002964097.1 NZ_CP007717:c229857-229543 [Brucella suis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46917 NZ_CP007717:c229857-229543 [Brucella suis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCGCCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA