Detailed information of TA system TA046916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-couple_hipB
• Location: 2366969..2367611
• Replicon: chromosome
• Accession: NZ_CP007715
• Organism: Actinobacillus equuli subsp. equuli strain 19392

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0GF68
• Locus tag: ACEE_RS11040
• Protein ID: WP_005621659.1
• Coordinates: 2366969..2367340 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: ACEE_RS11045
• Protein ID: WP_039198813.1
• Coordinates: 2367321..2367611 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACEE_RS11025	2363476..2364330	+	855	WP_039198806.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
ACEE_RS11030	2364374..2364943	-	570	WP_039198809.1	elongation factor P hydroxylase	-
ACEE_RS11035	2365003..2366751	-	1749	WP_039198811.1	protein-disulfide reductase DsbD	-
ACEE_RS11040	2366969..2367340	+	372	WP_005621659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACEE_RS11045	2367321..2367611	+	291	WP_039198813.1	helix-turn-helix transcriptional regulator	Antitoxin
ACEE_RS11050	2367679..2368641	-	963	WP_039198815.1	calcium/sodium antiporter	-
ACEE_RS11055	2368714..2369211	+	498	WP_039198817.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
ACEE_RS11060	2369436..2370170	+	735	WP_039198820.1	arginine ABC transporter ATP-binding protein ArtP	-
ACEE_RS11065	2370175..2370909	+	735	WP_197050930.1	transporter substrate-binding domain-containing protein	-
ACEE_RS11070	2370915..2371586	+	672	WP_039198825.1	arginine ABC transporter permease ArtQ	-
ACEE_RS11075	2371586..2372269	+	684	WP_039198827.1	arginine ABC transporter permease ArtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (33-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14938.10 Da
• Isoelectric Point: 9.5717

>T46916 WP_005621659.1 NZ_CP007715:2366969-2367340 [Actinobacillus equuli subsp. equuli]
MYEIVFYRDRRGREPVKEFLQEFINEQQDENRERLHKISHHLTILHLHGTRAGESYIKHLEDRIWQLRPISDCLLFAGIV
RGQFVLLHHFAKSSSRLPKRELERAKSRLADLQERIKDEPHWF

Nucleotide

• Length: 372 bp

>T46916 NZ_CP007715:2366969-2367340 [Actinobacillus equuli subsp. equuli]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACAATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10821.59 Da
• Isoelectric Point: 8.7275

>AT46916 WP_039198813.1 NZ_CP007715:2367321-2367611 [Actinobacillus equuli subsp. equuli]
MSPIGSSWTSFEREVFNELEMEENHFRVKLILEIIETRQALGISQRKLEKLSGVKQSMIARIEKGSSNPSLGTLLKLLVP
LGKTLQIVSLDETHSK

Nucleotide

• Length: 291 bp

>AT46916 NZ_CP007715:2367321-2367611 [Actinobacillus equuli subsp. equuli]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Structures

Toxin

• AlphaFold DB: A0A0A7MI46