Detailed information of TA system TA046906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 719687..720246
• Replicon: chromosome
• Accession: NZ_CP007695
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSSP1_RS03400
• Protein ID: WP_004688321.1
• Coordinates: 719687..719932 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSSP1_RS03405
• Protein ID: WP_002964097.1
• Coordinates: 719932..720246 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSSP1_RS03375	715534..717180	-	1647	WP_006072703.1	hypothetical protein	-
BSSP1_RS03380	717315..718088	-	774	WP_006072701.1	hypothetical protein	-
BSSP1_RS03395	718916..719554	-	639	Protein_664	tRNA-guanine transglycosylase	-
BSSP1_RS03400	719687..719932	+	246	WP_004688321.1	BrnT family toxin	Toxin
BSSP1_RS03405	719932..720246	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSSP1_RS03415	720594..720764	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
BSSP1_RS03420	721112..721822	-	711	WP_004683739.1	porin family protein	-
BSSP1_RS03425	722057..722644	-	588	WP_004683738.1	hypothetical protein	-
BSSP1_RS03430	722667..722942	-	276	WP_002964092.1	hypothetical protein	-
BSSP1_RS03435	722939..723169	-	231	WP_002964091.1	hypothetical protein	-
BSSP1_RS03440	723166..723885	-	720	WP_006072696.1	hypothetical protein	-
BSSP1_RS03445	723935..724138	+	204	WP_002964088.1	hypothetical protein	-
BSSP1_RS03450	724135..724350	+	216	WP_002971459.1	hypothetical protein	-
BSSP1_RS03455	724353..724556	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	712420..726328	13908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46906 WP_004688321.1 NZ_CP007695:719687-719932 [Brucella suis bv. 2]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46906 NZ_CP007695:719687-719932 [Brucella suis bv. 2]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46906 WP_002964097.1 NZ_CP007695:719932-720246 [Brucella suis bv. 2]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46906 NZ_CP007695:719932-720246 [Brucella suis bv. 2]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA