Detailed information of TA system TA046904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 719682..720241
• Replicon: chromosome
• Accession: NZ_CP007691
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSPT1_RS03395
• Protein ID: WP_004688321.1
• Coordinates: 719682..719927 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSPT1_RS03400
• Protein ID: WP_002964097.1
• Coordinates: 719927..720241 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSPT1_RS03370	715529..717175	-	1647	WP_006072703.1	hypothetical protein	-
BSPT1_RS03375	717310..718083	-	774	WP_006072701.1	hypothetical protein	-
BSPT1_RS03390	718911..719549	-	639	Protein_664	tRNA-guanine transglycosylase	-
BSPT1_RS03395	719682..719927	+	246	WP_004688321.1	BrnT family toxin	Toxin
BSPT1_RS03400	719927..720241	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSPT1_RS03405	720589..720759	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
BSPT1_RS03410	721107..721817	-	711	WP_004683739.1	porin family protein	-
BSPT1_RS03415	722052..722639	-	588	WP_004683738.1	hypothetical protein	-
BSPT1_RS03420	722662..722937	-	276	WP_002964092.1	hypothetical protein	-
BSPT1_RS03425	722934..723164	-	231	WP_002964091.1	hypothetical protein	-
BSPT1_RS03430	723161..723880	-	720	WP_006072696.1	hypothetical protein	-
BSPT1_RS03435	723930..724133	+	204	WP_002964088.1	hypothetical protein	-
BSPT1_RS03440	724130..724345	+	216	WP_002971459.1	hypothetical protein	-
BSPT1_RS03445	724348..724551	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	712415..726335	13920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46904 WP_004688321.1 NZ_CP007691:719682-719927 [Brucella suis bv. 2]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46904 NZ_CP007691:719682-719927 [Brucella suis bv. 2]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46904 WP_002964097.1 NZ_CP007691:719927-720241 [Brucella suis bv. 2]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46904 NZ_CP007691:719927-720241 [Brucella suis bv. 2]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA