Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2208167..2208681 Replicon chromosome
Accession NZ_CP007690
Organism Staphylococcus aureus strain UA-S391_USA300 isolate abscess/wound isolate

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag EX97_RS11250 Protein ID WP_000581792.1
Coordinates 2208167..2208376 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag EX97_RS11255 Protein ID WP_000138932.1
Coordinates 2208388..2208681 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EX97_RS11230 2203675..2205033 - 1359 WP_000611462.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
EX97_RS11235 2205048..2206118 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
EX97_RS11240 2206436..2207638 + 1203 WP_001109939.1 rod shape-determining protein RodA -
EX97_RS11245 2207902..2208039 - 138 WP_000828354.1 hypothetical protein -
EX97_RS11250 2208167..2208376 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
EX97_RS11255 2208388..2208681 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
EX97_RS11260 2208847..2210331 + 1485 WP_000571549.1 cardiolipin synthase -
EX97_RS11265 2210359..2211006 + 648 WP_001187629.1 HD domain-containing protein -
EX97_RS11280 2211819..2212691 - 873 WP_000725802.1 membrane protein insertase YidC -
EX97_RS11285 2212777..2213418 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T46897 WP_000581792.1 NZ_CP007690:c2208376-2208167 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T46897 NZ_CP007690:c2208376-2208167 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT46897 WP_000138932.1 NZ_CP007690:c2208681-2208388 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT46897 NZ_CP007690:c2208681-2208388 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References