Detailed information of TA system TA046878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2157801..2158330
• Replicon: chromosome
• Accession: NZ_CP007676
• Organism: Staphylococcus aureus strain HUV05

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ER12_RS10990
• Protein ID: WP_000621175.1
• Coordinates: 2157801..2158163 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ER12_RS10995
• Protein ID: WP_000948331.1
• Coordinates: 2158160..2158330 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER12_RS10965	2154779..2155549	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
ER12_RS10970	2155524..2156003	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
ER12_RS10975	2156005..2156331	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ER12_RS10980	2156450..2157451	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ER12_RS10990	2157801..2158163	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ER12_RS10995	2158160..2158330	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ER12_RS11000	2158415..2159563	-	1149	WP_001281145.1	alanine racemase	-
ER12_RS11005	2159629..2159988	-	360	WP_000581200.1	holo-ACP synthase	-
ER12_RS11010	2159992..2160483	-	492	WP_001205910.1	PH domain-containing protein	-
ER12_RS11015	2160470..2162053	-	1584	WP_001294626.1	PH domain-containing protein	-
ER12_RS11020	2162046..2162525	-	480	WP_001287088.1	hypothetical protein	-
ER12_RS11025	2162733..2163293	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46878 WP_000621175.1 NZ_CP007676:c2158163-2157801 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46878 NZ_CP007676:c2158163-2157801 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46878 WP_000948331.1 NZ_CP007676:c2158330-2158160 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46878 NZ_CP007676:c2158330-2158160 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1