Detailed information of TA system TA046846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2157135..2157664
• Replicon: chromosome
• Accession: NZ_CP007672
• Organism: Staphylococcus aureus strain CA12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EQ80_RS10995
• Protein ID: WP_000621175.1
• Coordinates: 2157135..2157497 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EQ80_RS11000
• Protein ID: WP_000948331.1
• Coordinates: 2157494..2157664 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ80_RS10970	2154113..2154883	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
EQ80_RS10975	2154858..2155337	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EQ80_RS10980	2155339..2155665	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EQ80_RS10985	2155784..2156785	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EQ80_RS10995	2157135..2157497	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EQ80_RS11000	2157494..2157664	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EQ80_RS11005	2157749..2158897	-	1149	WP_001281145.1	alanine racemase	-
EQ80_RS11010	2158963..2159322	-	360	WP_000581200.1	holo-ACP synthase	-
EQ80_RS11015	2159326..2159817	-	492	WP_001205910.1	PH domain-containing protein	-
EQ80_RS11020	2159804..2161387	-	1584	WP_001294626.1	PH domain-containing protein	-
EQ80_RS11025	2161380..2161859	-	480	WP_001287088.1	hypothetical protein	-
EQ80_RS11030	2162067..2162627	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46846 WP_000621175.1 NZ_CP007672:c2157497-2157135 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46846 NZ_CP007672:c2157497-2157135 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46846 WP_000948331.1 NZ_CP007672:c2157664-2157494 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46846 NZ_CP007672:c2157664-2157494 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1