Detailed information of TA system TA046830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2213090..2213619
• Replicon: chromosome
• Accession: NZ_CP007670
• Organism: Staphylococcus aureus strain M121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EP54_RS11435
• Protein ID: WP_000621175.1
• Coordinates: 2213090..2213452 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EP54_RS11440
• Protein ID: WP_000948331.1
• Coordinates: 2213449..2213619 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP54_RS11410	2210068..2210838	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
EP54_RS11415	2210813..2211292	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EP54_RS11420	2211294..2211620	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EP54_RS11425	2211739..2212740	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EP54_RS11435	2213090..2213452	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EP54_RS11440	2213449..2213619	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EP54_RS11445	2213704..2214852	-	1149	WP_001281145.1	alanine racemase	-
EP54_RS11450	2214918..2215277	-	360	WP_000581200.1	holo-ACP synthase	-
EP54_RS11455	2215281..2215772	-	492	WP_001205910.1	PH domain-containing protein	-
EP54_RS11460	2215759..2217342	-	1584	WP_001294626.1	PH domain-containing protein	-
EP54_RS11465	2217335..2217814	-	480	WP_001287088.1	hypothetical protein	-
EP54_RS11470	2218022..2218582	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46830 WP_000621175.1 NZ_CP007670:c2213452-2213090 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46830 NZ_CP007670:c2213452-2213090 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46830 WP_000948331.1 NZ_CP007670:c2213619-2213449 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46830 NZ_CP007670:c2213619-2213449 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1