Detailed information of TA system TA046807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2165061..2165590
• Replicon: chromosome
• Accession: NZ_CP007659
• Organism: Staphylococcus aureus strain H-EMRSA-15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ER16_RS10965
• Protein ID: WP_000621175.1
• Coordinates: 2165061..2165423 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ER16_RS10970
• Protein ID: WP_000948330.1
• Coordinates: 2165420..2165590 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER16_RS10940	2162039..2162809	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
ER16_RS10945	2162784..2163263	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
ER16_RS10950	2163265..2163591	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ER16_RS10955	2163711..2164712	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ER16_RS10965	2165061..2165423	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ER16_RS10970	2165420..2165590	-	171	WP_000948330.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ER16_RS10975	2165675..2166823	-	1149	WP_001281141.1	alanine racemase	-
ER16_RS10980	2166889..2167248	-	360	WP_000581193.1	holo-ACP synthase	-
ER16_RS10985	2167252..2167743	-	492	WP_001205918.1	PH domain-containing protein	-
ER16_RS10990	2167730..2169313	-	1584	WP_001294624.1	PH domain-containing protein	-
ER16_RS10995	2169306..2169785	-	480	WP_001287080.1	hypothetical protein	-
ER16_RS11000	2169994..2170554	-	561	WP_001092400.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46807 WP_000621175.1 NZ_CP007659:c2165423-2165061 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46807 NZ_CP007659:c2165423-2165061 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6237.75 Da
• Isoelectric Point: 3.8305

>AT46807 WP_000948330.1 NZ_CP007659:c2165590-2165420 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNESYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46807 NZ_CP007659:c2165590-2165420 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA