Detailed information of TA system TA046787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2147216..2147745
• Replicon: chromosome
• Accession: NZ_CP007657
• Organism: Staphylococcus aureus strain V2200

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EP20_RS10920
• Protein ID: WP_000621175.1
• Coordinates: 2147216..2147578 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EP20_RS10925
• Protein ID: WP_000948331.1
• Coordinates: 2147575..2147745 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP20_RS10895	2144194..2144964	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
EP20_RS10900	2144939..2145418	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EP20_RS10905	2145420..2145746	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EP20_RS10910	2145865..2146866	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EP20_RS10920	2147216..2147578	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EP20_RS10925	2147575..2147745	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EP20_RS10930	2147830..2148978	-	1149	WP_001281145.1	alanine racemase	-
EP20_RS10935	2149044..2149403	-	360	WP_000581200.1	holo-ACP synthase	-
EP20_RS10940	2149407..2149898	-	492	WP_001205910.1	PH domain-containing protein	-
EP20_RS10945	2149885..2151468	-	1584	WP_001294626.1	PH domain-containing protein	-
EP20_RS10950	2151461..2151940	-	480	WP_001287088.1	hypothetical protein	-
EP20_RS10955	2152148..2152708	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46787 WP_000621175.1 NZ_CP007657:c2147578-2147216 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46787 NZ_CP007657:c2147578-2147216 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46787 WP_000948331.1 NZ_CP007657:c2147745-2147575 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46787 NZ_CP007657:c2147745-2147575 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1