Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 670024..670709 Replicon plasmid pRetIE4803d
Accession NZ_CP007645
Organism Rhizobium etli bv. phaseoli str. IE4803

Toxin (Protein)


Gene name vapC Uniprot ID A0A060IDY5
Locus tag IE4803_RS32625 Protein ID WP_040113056.1
Coordinates 670287..670709 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7U4IYE0
Locus tag IE4803_RS32620 Protein ID WP_040113055.1
Coordinates 670024..670290 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE4803_RS32600 665639..666691 + 1053 WP_040113051.1 iron ABC transporter permease -
IE4803_RS32605 666688..667476 + 789 WP_040113052.1 ABC transporter ATP-binding protein -
IE4803_RS32610 667505..667933 - 429 WP_040113053.1 MarR family transcriptional regulator -
IE4803_RS32615 668140..669951 + 1812 WP_040113054.1 chloride channel protein -
IE4803_RS32620 670024..670290 + 267 WP_040113055.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IE4803_RS32625 670287..670709 + 423 WP_040113056.1 type II toxin-antitoxin system VapC family toxin Toxin
IE4803_RS32630 670735..671505 - 771 WP_040113057.1 ABC transporter ATP-binding protein -
IE4803_RS32635 671515..672498 - 984 WP_040113058.1 ABC transporter substrate-binding protein -
IE4803_RS32640 672537..673388 - 852 WP_040113059.1 ABC transporter permease -
IE4803_RS32645 673399..674751 - 1353 WP_040113060.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..742914 742914


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-63)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15617.02 Da        Isoelectric Point: 4.6791

>T46778 WP_040113056.1 NZ_CP007645:670287-670709 [Rhizobium etli bv. phaseoli str. IE4803]
VRLLLDTNVLLEVTKPNPEMRVLAWLDKLDEDRSFISIVSVAEIRRGVALMDEGRKRDALAEWLARDLPQRFEGRIIPMD
EPVALAWGDLMGPAKRNGRGLSSIDGLIAATAVAHDLTLATRNTRDFEGFGIELIDPWIA

Download         Length: 423 bp

>T46778 NZ_CP007645:670287-670709 [Rhizobium etli bv. phaseoli str. IE4803]
GTGAGGCTTCTGCTGGACACCAACGTGCTGTTGGAGGTGACGAAGCCGAACCCCGAAATGCGCGTCTTGGCTTGGCTGGA
TAAGCTGGATGAGGATCGCTCCTTCATCAGCATCGTGTCTGTCGCGGAGATCCGGCGCGGTGTTGCCCTGATGGACGAGG
GACGAAAACGCGACGCGCTGGCCGAATGGCTTGCCCGGGACCTGCCGCAACGCTTCGAGGGCCGCATCATTCCGATGGAC
GAGCCGGTTGCCTTAGCCTGGGGTGACCTTATGGGCCCCGCGAAGCGGAACGGCCGCGGCCTGTCGTCGATAGATGGACT
GATCGCTGCAACCGCGGTTGCTCACGATCTCACTCTCGCCACGCGCAACACCAGGGATTTCGAGGGGTTCGGGATCGAAC
TCATCGATCCCTGGATCGCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9834.04 Da        Isoelectric Point: 8.7841

>AT46778 WP_040113055.1 NZ_CP007645:670024-670290 [Rhizobium etli bv. phaseoli str. IE4803]
MRPSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARKGTLAEFLMSSPLRGADLDLERQR
DDPRDPEL

Download         Length: 267 bp

>AT46778 NZ_CP007645:670024-670290 [Rhizobium etli bv. phaseoli str. IE4803]
ATGCGACCGTCACGGCAAGGCGATGAAAGCTGGACCGTCGCCAGCGCAAAGGCAAAGCTTTCCGAGGTTATCGAACGCGC
GCAATCCGCGCCGCAAACGATTACCCGGAACGGCAAGCCGAGCGTCGTCGTCGTCTCCGCCGAGGAGTGGCAGCGCAAGA
CTGCGCGCAAGGGTACGCTTGCCGAATTCCTGATGAGCTCGCCGCTGCGTGGCGCCGATCTGGATCTCGAGCGTCAACGC
GACGATCCGCGTGATCCCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060IDY5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4IYE0

References