Detailed information of TA system TA046742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 942578..943137
• Replicon: chromosome
• Accession: NZ_CP007629
• Organism: Brucella canis strain SVA13

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DA85_RS04525
• Protein ID: WP_004688321.1
• Coordinates: 942892..943137 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: DA85_RS04520
• Protein ID: WP_002964097.1
• Coordinates: 942578..942892 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA85_RS04475	938268..938471	-	204	WP_004690812.1	hypothetical protein	-
DA85_RS04480	938474..938689	-	216	WP_002971459.1	hypothetical protein	-
DA85_RS04485	938686..938889	-	204	WP_002964088.1	hypothetical protein	-
DA85_RS04490	938939..939658	+	720	WP_006072696.1	hypothetical protein	-
DA85_RS04495	939655..939885	+	231	WP_002964091.1	hypothetical protein	-
DA85_RS04500	939882..940157	+	276	WP_002964092.1	hypothetical protein	-
DA85_RS04505	940180..940767	+	588	WP_004683738.1	hypothetical protein	-
DA85_RS04510	941002..941712	+	711	WP_004683739.1	porin family protein	-
DA85_RS04515	942060..942230	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DA85_RS04520	942578..942892	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
DA85_RS04525	942892..943137	-	246	WP_004688321.1	BrnT family toxin	Toxin
DA85_RS04530	943270..943908	+	639	Protein_891	tRNA-guanine transglycosylase	-
DA85_RS17340	943978..944735	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
DA85_RS04545	944736..945509	+	774	WP_006132649.1	hypothetical protein	-
DA85_RS04550	945644..947290	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	936496..950606	14110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T46742 WP_004688321.1 NZ_CP007629:c943137-942892 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T46742 NZ_CP007629:c943137-942892 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT46742 WP_002964097.1 NZ_CP007629:c942892-942578 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT46742 NZ_CP007629:c942892-942578 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA