Detailed information of TA system TA046740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2697300..2697942
• Replicon: chromosome
• Accession: NZ_CP007626
• Organism: Bacillus pseudomycoides strain 219298

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: R8PUN4
• Locus tag: MA06_RS16165
• Protein ID: WP_003194532.1
• Coordinates: 2697592..2697942 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R8PTJ6
• Locus tag: MA06_RS16160
• Protein ID: WP_000004571.1
• Coordinates: 2697300..2697587 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA06_RS16135	2692533..2693495	+	963	WP_018782019.1	UV DNA damage repair endonuclease UvsE	-
MA06_RS29345	2693499..2694059	-	561	WP_042511364.1	rhomboid family intramembrane serine protease	-
MA06_RS16145	2694152..2694511	+	360	WP_006093158.1	holo-ACP synthase	-
MA06_RS16150	2694668..2695615	+	948	WP_029427332.1	outer membrane lipoprotein carrier protein LolA	-
MA06_RS16155	2695882..2697051	+	1170	WP_040119081.1	alanine racemase	-
MA06_RS16160	2697300..2697587	+	288	WP_000004571.1	antitoxin EndoAI	Antitoxin
MA06_RS16165	2697592..2697942	+	351	WP_003194532.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MA06_RS16170	2698008..2700176	+	2169	WP_018782023.1	RNA-binding transcriptional accessory protein	-
MA06_RS30445	2700286..2700402	-	117	WP_017154227.1	cortex morphogenetic protein CmpA	-
MA06_RS16175	2700657..2701142	+	486	WP_018767122.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12980.10 Da
• Isoelectric Point: 5.7168

>T46740 WP_003194532.1 NZ_CP007626:2697592-2697942 [Bacillus pseudomycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEMMMSRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T46740 NZ_CP007626:2697592-2697942 [Bacillus pseudomycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACACATGTGGAAATCGATGCAAAAAAGTACGGATTTGAGAGAGATTCTGTTATCTTGCTCGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAGATGATGATGAGTCGTGTAGACGAAGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10748.30 Da
• Isoelectric Point: 8.9605

>AT46740 WP_000004571.1 NZ_CP007626:2697300-2697587 [Bacillus pseudomycoides]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Nucleotide

• Length: 288 bp

>AT46740 NZ_CP007626:2697300-2697587 [Bacillus pseudomycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: R8PUN4

Antitoxin

• AlphaFold DB: R8PTJ6