Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 4052355..4053238 Replicon chromosome
Accession NZ_CP007620
Organism Pseudomonas putida strain DLL-E4

Toxin (Protein)


Gene name - Uniprot ID A0A0C1HTG7
Locus tag DW66_RS18690 Protein ID WP_015270851.1
Coordinates 4052355..4052792 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID F8G488
Locus tag DW66_RS18695 Protein ID WP_013973163.1
Coordinates 4052789..4053238 (-) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW66_RS18675 4048725..4049954 - 1230 WP_013973159.1 acyl-CoA dehydrogenase -
DW66_RS18680 4050095..4051021 + 927 WP_013973160.1 LysR family transcriptional regulator -
DW66_RS18685 4051193..4052347 + 1155 WP_013973161.1 aminotransferase class V-fold PLP-dependent enzyme -
DW66_RS18690 4052355..4052792 - 438 WP_015270851.1 RES family NAD+ phosphorylase Toxin
DW66_RS18695 4052789..4053238 - 450 WP_013973163.1 DUF2384 domain-containing protein Antitoxin
DW66_RS18700 4053337..4053990 - 654 WP_013973164.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
DW66_RS18705 4054095..4055018 + 924 WP_038409932.1 LysR family transcriptional regulator -
DW66_RS18710 4055066..4055896 + 831 WP_013973166.1 AraC family transcriptional regulator -
DW66_RS18715 4055947..4056531 + 585 WP_013973167.1 LysE family translocator -
DW66_RS18720 4056658..4057872 - 1215 WP_024087364.1 sugar transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-137)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15969.19 Da        Isoelectric Point: 5.1199

>T46739 WP_015270851.1 NZ_CP007620:c4052792-4052355 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSSQTELTRGLGNRFLDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Download         Length: 438 bp

>T46739 NZ_CP007620:c4052792-4052355 [Pseudomonas putida]
GTGATTCTGTGGCGTATCAGCGCCTATGCAGACTTGAGCGGAACGGGCGGCTTGCGTGTCAGCGGCCGCTGGCACCAGGC
GGGCCGGCCAGTGGTGTATGCCGCCACCAGCCCGGCCGGGGCGATGCTGGAGGTGCTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCTGACACCGTTTCGCAAGCGCAGTTGCCCGCCTTGCAG
CCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGCTTTCTGGATGAGTGTTCGGCGTTGCTGCTGCC
GGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTTTTCAACCCGCGCCATCCGCAGGCAAACAGTGCCAGGCTCG
AGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 17004.59 Da        Isoelectric Point: 6.3405

>AT46739 WP_013973163.1 NZ_CP007620:c4053238-4052789 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Download         Length: 450 bp

>AT46739 NZ_CP007620:c4053238-4052789 [Pseudomonas putida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCCTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGCGACCGGCTGTTCCGTTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCAGGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTAACCACCCAGCAGGGCACCA
CCCAGGTCGAAGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C1HTG7

Antitoxin

Source ID Structure
AlphaFold DB F8G488

References