Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 3949538..3950231 Replicon chromosome
Accession NZ_CP007620
Organism Pseudomonas putida strain DLL-E4

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag DW66_RS18155 Protein ID WP_003151133.1
Coordinates 3949538..3949915 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag DW66_RS18160 Protein ID WP_001172026.1
Coordinates 3949896..3950231 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW66_RS18135 3944597..3945118 + 522 WP_049870827.1 cupin domain-containing protein -
DW66_RS32740 3945198..3945443 + 246 WP_038409896.1 hypothetical protein -
DW66_RS18145 3945733..3948760 - 3028 Protein_3613 Tn3 family transposase -
DW66_RS18150 3948744..3949346 - 603 WP_010465829.1 recombinase family protein -
DW66_RS18155 3949538..3949915 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DW66_RS18160 3949896..3950231 + 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
DW66_RS18165 3950246..3950581 + 336 WP_000741275.1 hypothetical protein -
DW66_RS18170 3950605..3950931 + 327 WP_000091614.1 hypothetical protein -
DW66_RS18175 3950928..3951299 + 372 WP_023294894.1 hypothetical protein -
DW66_RS18180 3951339..3952163 + 825 Protein_3620 MFS transporter -
DW66_RS18185 3952188..3953264 - 1077 WP_038409898.1 NAD(P)/FAD-dependent oxidoreductase -
DW66_RS18190 3953347..3953736 - 390 WP_038409899.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3925897..3961182 35285
- inside IScluster/Tn - - 3945733..3960897 15164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T46738 WP_003151133.1 NZ_CP007620:3949538-3949915 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T46738 NZ_CP007620:3949538-3949915 [Pseudomonas putida]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT46738 WP_001172026.1 NZ_CP007620:3949896-3950231 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT46738 NZ_CP007620:3949896-3950231 [Pseudomonas putida]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References