Detailed information of TA system TA046737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2543891..2544407
• Replicon: chromosome
• Accession: NZ_CP007620
• Organism: Pseudomonas putida strain DLL-E4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: DW66_RS11815
• Protein ID: WP_003259987.1
• Coordinates: 2544126..2544407 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: DW66_RS11810
• Protein ID: WP_013972043.1
• Coordinates: 2543891..2544136 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DW66_RS11790	2539318..2539860	-	543	WP_038409286.1	WYL domain-containing protein	-
DW66_RS11795	2539968..2540954	-	987	WP_013972040.1	zinc-binding dehydrogenase	-
DW66_RS11800	2541317..2542510	+	1194	WP_038409288.1	MFS transporter	-
DW66_RS11805	2542541..2543647	+	1107	WP_031325095.1	alkene reductase	-
DW66_RS11810	2543891..2544136	+	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DW66_RS11815	2544126..2544407	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DW66_RS11820	2544446..2544841	-	396	WP_023662326.1	hypothetical protein	-
DW66_RS11825	2544930..2545838	-	909	WP_038409290.1	LysR family transcriptional regulator	-
DW66_RS11830	2546130..2547434	+	1305	WP_013972046.1	MHS family MFS transporter	-
DW66_RS11835	2547465..2548706	+	1242	WP_013972047.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T46737 WP_003259987.1 NZ_CP007620:2544126-2544407 [Pseudomonas putida]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T46737 NZ_CP007620:2544126-2544407 [Pseudomonas putida]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGGATCGTTGTAGTGGTGGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT46737 WP_013972043.1 NZ_CP007620:2543891-2544136 [Pseudomonas putida]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT46737 NZ_CP007620:2543891-2544136 [Pseudomonas putida]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AACGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTATCCCTGGATGAC
CTATAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6