Detailed information of TA system TA046733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 97594..98236
• Replicon: chromosome
• Accession: NZ_CP007607
• Organism: Bacillus thuringiensis serovar kurstaki str. YBT-1520

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: R8I8B8
• Locus tag: DF16_RS00590
• Protein ID: WP_000635965.1
• Coordinates: 97594..97944 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: DF16_RS00595
• Protein ID: WP_038415080.1
• Coordinates: 97949..98236 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DF16_RS00575	94529..94987	-	459	WP_000344239.1	SprT family protein	-
DF16_RS00580	95183..95299	+	117	WP_001143642.1	cortex morphogenetic protein CmpA	-
DF16_RS00585	95358..97526	-	2169	WP_000426236.1	RNA-binding transcriptional accessory protein	-
DF16_RS00590	97594..97944	-	351	WP_000635965.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DF16_RS00595	97949..98236	-	288	WP_038415080.1	hypothetical protein	Antitoxin
DF16_RS00600	98545..99714	-	1170	WP_000390616.1	alanine racemase	-
DF16_RS00605	99832..100782	-	951	WP_002100659.1	outer membrane lipoprotein carrier protein LolA	-
DF16_RS00610	100939..101298	-	360	WP_000583417.1	holo-ACP synthase	-
DF16_RS00615	101392..101964	+	573	WP_038415082.1	rhomboid family intramembrane serine protease	-
DF16_RS00620	101957..102919	-	963	WP_000961167.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12948.04 Da
• Isoelectric Point: 5.7168

>T46733 WP_000635965.1 NZ_CP007607:c97944-97594 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T46733 NZ_CP007607:c97944-97594 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10948.60 Da
• Isoelectric Point: 9.9227

>AT46733 WP_038415080.1 NZ_CP007607:c98236-97949 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVRRG

Nucleotide

• Length: 288 bp

>AT46733 NZ_CP007607:c98236-97949 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGGCGGGGGTAA

Structures

Toxin

• AlphaFold DB: A0A366G118