Detailed information of TA system TA046727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-MazE
• Location: 37020..37594
• Replicon: plasmid pAYP1020
• Accession: NZ_CP007602
• Organism: Staphylococcus capitis subsp. capitis strain AYP1020

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ayp1020_RS11855
• Protein ID: WP_047797159.1
• Coordinates: 37020..37355 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: ayp1020_RS11860
• Protein ID: WP_047797160.1
• Coordinates: 37349..37594 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ayp1020_RS11805	32031..32255	-	225	WP_047797149.1	hypothetical protein	-
ayp1020_RS11810	32258..32482	-	225	WP_047797150.1	hypothetical protein	-
ayp1020_RS11815	32572..33153	-	582	WP_047797151.1	hypothetical protein	-
ayp1020_RS11820	33219..33824	-	606	WP_047797152.1	hypothetical protein	-
ayp1020_RS11825	33935..34291	-	357	WP_047797153.1	hypothetical protein	-
ayp1020_RS11830	34309..34515	-	207	WP_047797154.1	hypothetical protein	-
ayp1020_RS11835	34531..34722	-	192	WP_047797155.1	DUF4314 domain-containing protein	-
ayp1020_RS12360	34820..34990	-	171	WP_158491160.1	hypothetical protein	-
ayp1020_RS11840	35390..35779	-	390	WP_047797156.1	hypothetical protein	-
ayp1020_RS11845	35854..36249	-	396	WP_144427335.1	hypothetical protein	-
ayp1020_RS11850	36378..36887	-	510	WP_047797158.1	hypothetical protein	-
ayp1020_RS11855	37020..37355	-	336	WP_047797159.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ayp1020_RS11860	37349..37594	-	246	WP_047797160.1	hypothetical protein	Antitoxin
ayp1020_RS11865	37662..38099	-	438	WP_047797161.1	single-stranded DNA-binding protein	-
ayp1020_RS12005	38128..38790	-	663	WP_053035568.1	hypothetical protein	-
ayp1020_RS11875	38886..39338	-	453	WP_047797162.1	hypothetical protein	-
ayp1020_RS11880	39357..39566	-	210	WP_047797163.1	hypothetical protein	-
ayp1020_RS12365	39698..39859	-	162	WP_158491161.1	hypothetical protein	-
ayp1020_RS11885	39977..40222	-	246	WP_047797164.1	hypothetical protein	-
ayp1020_RS11890	40222..41070	-	849	WP_047797165.1	ParA family protein	-
ayp1020_RS11895	41063..41374	-	312	WP_047797166.1	hypothetical protein	-
ayp1020_RS11900	41508..41735	-	228	WP_047797167.1	hypothetical protein	-
ayp1020_RS11905	42218..42484	-	267	WP_047797168.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59661	59661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12841.88 Da
• Isoelectric Point: 9.9723

>T46727 WP_047797159.1 NZ_CP007602:c37355-37020 [Staphylococcus capitis subsp. capitis]
IMINQFDVIMIDLNPTRGKEKQKYRPCVVVSNNFVNQHSPFVWILPITNRPKRFPSDISIETKNNSVTGIIDSIQIRSLD
VKVRNYKKIDELNENIKQDVIHTIIAHSSIV

Nucleotide

• Length: 336 bp

>T46727 NZ_CP007602:c37355-37020 [Staphylococcus capitis subsp. capitis]
ATTATGATAAATCAATTTGATGTGATCATGATTGATTTAAATCCTACACGTGGCAAAGAAAAACAAAAGTATCGACCATG
TGTAGTTGTAAGCAACAATTTTGTAAATCAACATTCCCCGTTCGTATGGATTTTACCTATAACTAACAGACCTAAAAGAT
TTCCATCAGATATATCTATTGAAACTAAAAATAATAGCGTGACAGGTATCATTGATTCTATACAAATTAGGTCTTTAGAT
GTGAAAGTTAGAAACTATAAGAAGATTGATGAATTGAATGAAAACATTAAACAGGATGTAATTCATACAATTATTGCTCA
TTCAAGCATTGTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9024.89 Da
• Isoelectric Point: 4.2086

>AT46727 WP_047797160.1 NZ_CP007602:c37594-37349 [Staphylococcus capitis subsp. capitis]
MSQLAKVIKNGNSQAITLNKNVLNESGLNIGDSLSVEVNDGEIKFTKNNKSIIDEIHDFYRNGGYYDEEEIDFGDSVGRE
L

Nucleotide

• Length: 246 bp

>AT46727 NZ_CP007602:c37594-37349 [Staphylococcus capitis subsp. capitis]
ATGAGTCAATTAGCTAAAGTAATTAAAAATGGTAATAGTCAAGCAATCACTTTAAATAAGAATGTTTTAAATGAATCAGG
TTTAAATATCGGTGATAGTTTATCGGTAGAAGTTAATGATGGTGAAATTAAATTTACAAAAAATAATAAATCAATTATAG
ATGAAATTCATGATTTTTATCGTAATGGTGGATATTACGATGAGGAAGAAATTGATTTTGGAGACTCAGTAGGTCGAGAA
TTATGA