Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5050035..5050611 Replicon chromosome
Accession NZ_CP007594
Organism Escherichia coli strain SEC470

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag DP32_RS25380 Protein ID WP_001297643.1
Coordinates 5050035..5050322 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag DP32_RS25385 Protein ID WP_000063156.1
Coordinates 5050309..5050611 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DP32_RS25360 5046288..5047568 - 1281 WP_001037380.1 DUF445 domain-containing protein -
DP32_RS25365 5047609..5048841 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
DP32_RS28215 5048987..5049082 - 96 Protein_4798 dienelactone hydrolase family protein -
DP32_RS28890 5049139..5049836 + 698 Protein_4799 IS1 family transposase -
DP32_RS25380 5050035..5050322 + 288 WP_001297643.1 BrnT family toxin Toxin
DP32_RS25385 5050309..5050611 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
DP32_RS25390 5050645..5051601 - 957 WP_001297640.1 GTPase -
DP32_RS25395 5051612..5051815 - 204 WP_000467859.1 YbdD/YjiX family protein -
DP32_RS25400 5051865..5054015 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimB / fimE / fimA / fimI / fimC / fimD / fimF / fimG / fimH / cheD 5000309..5147778 147469


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T46713 WP_001297643.1 NZ_CP007594:5050035-5050322 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T46713 NZ_CP007594:5050035-5050322 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT46713 WP_000063156.1 NZ_CP007594:5050309-5050611 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT46713 NZ_CP007594:5050309-5050611 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References