Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3206687..3207270 Replicon chromosome
Accession NZ_CP007594
Organism Escherichia coli strain SEC470

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag DP32_RS16150 Protein ID WP_000254738.1
Coordinates 3206687..3207022 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID E3PHU0
Locus tag DP32_RS16155 Protein ID WP_000581938.1
Coordinates 3207022..3207270 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DP32_RS16135 3202574..3203872 - 1299 WP_000036723.1 phosphopyruvate hydratase -
DP32_RS16140 3203960..3205597 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
DP32_RS16145 3205825..3206616 - 792 WP_001071653.1 nucleoside triphosphate pyrophosphohydrolase -
DP32_RS16150 3206687..3207022 - 336 WP_000254738.1 endoribonuclease MazF Toxin
DP32_RS16155 3207022..3207270 - 249 WP_000581938.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DP32_RS16160 3207348..3209582 - 2235 WP_000226815.1 GTP pyrophosphokinase -
DP32_RS16165 3209630..3210931 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T46703 WP_000254738.1 NZ_CP007594:c3207022-3206687 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T46703 NZ_CP007594:c3207022-3206687 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9403.82 Da        Isoelectric Point: 4.5972

>AT46703 WP_000581938.1 NZ_CP007594:c3207270-3207022 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKYKE
VW

Download         Length: 249 bp

>AT46703 NZ_CP007594:c3207270-3207022 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAATATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB E3PHU0

References