Detailed information of TA system TA046684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1004288..1005543
• Replicon: chromosome
• Accession: NZ_CP007593
• Organism: Streptococcus pneumoniae strain NT_110_58

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A0B4ZH99
• Locus tag: spnnt_RS04960
• Protein ID: WP_039704000.1
• Coordinates: 1004764..1005543 (+)
• Length: 260 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: spnnt_RS04955
• Protein ID: WP_025173761.1
• Coordinates: 1004288..1004764 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
spnnt_RS04925	999695..1000965	-	1271	Protein_953	IS110 family transposase	-
spnnt_RS04930	1001508..1002149	+	642	WP_016397477.1	hypothetical protein	-
spnnt_RS04935	1002159..1003244	+	1086	Protein_955	toprim domain-containing protein	-
spnnt_RS04940	1003295..1003528	+	234	WP_000152423.1	hypothetical protein	-
spnnt_RS04945	1003525..1003908	+	384	WP_002933294.1	hypothetical protein	-
spnnt_RS04950	1003946..1004218	+	273	WP_016399544.1	hypothetical protein	-
spnnt_RS04955	1004288..1004764	+	477	WP_025173761.1	helix-turn-helix domain-containing protein	Antitoxin
spnnt_RS04960	1004764..1005543	+	780	WP_039704000.1	zeta toxin family protein	Toxin
spnnt_RS04965	1005545..1006941	+	1397	Protein_961	hypothetical protein	-
spnnt_RS04970	1006943..1007638	+	696	WP_016399482.1	hypothetical protein	-
spnnt_RS04975	1007635..1010283	+	2649	WP_016399481.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	981499..1028694	47195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 260 a.a.
• Molecular weight: 29613.54 Da
• Isoelectric Point: 6.5044

>T46684 WP_039704000.1 NZ_CP007593:1004764-1005543 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKETAQLLKSKGYEVQLALIATKPELSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSGEDTTSAATVLHDLLFGEWNTVEKEML
KIGQERLWVINKELNNRGD

Nucleotide

• Length: 780 bp

>T46684 NZ_CP007593:1004764-1005543 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCTGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACGATTGACGTTCCAAAGGAAACAGCACAACTCTTGAAAAGTA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAGCCTGAGCTGTCCTATCTGAGCACTCTTATCCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGGCAATTAGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAG
GAGAAGATACAACTTCAGCAGCAACAGTTCTTCATGACTTACTATTTGGAGAATGGAACACGGTTGAAAAAGAGATGTTG
AAGATAGGGCAGGAACGGTTGTGGGTGATTAACAAAGAGTTAAATAATAGGGGGGATTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18279.70 Da
• Isoelectric Point: 4.4188

>AT46684 WP_025173761.1 NZ_CP007593:1004288-1004764 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMFNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT46684 NZ_CP007593:1004288-1004764 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAACTAATAGATATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGTTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0B4ZH99