Detailed information of TA system TA046682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 666053..667311
• Replicon: chromosome
• Accession: NZ_CP007593
• Organism: Streptococcus pneumoniae strain NT_110_58

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: A0A064C2H2
• Locus tag: spnnt_RS03145
• Protein ID: WP_016569769.1
• Coordinates: 666529..667311 (+)
• Length: 261 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: spnnt_RS03140
• Protein ID: WP_016569768.1
• Coordinates: 666053..666529 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
spnnt_RS03110	661709..662965	-	1257	WP_023930580.1	ISL3 family transposase	-
spnnt_RS03115	663207..663845	+	639	WP_016569764.1	hypothetical protein	-
spnnt_RS03120	663884..664972	+	1089	WP_016569765.1	toprim domain-containing protein	-
spnnt_RS03125	665026..665259	+	234	WP_016569766.1	hypothetical protein	-
spnnt_RS03130	665256..665651	+	396	WP_023928737.1	hypothetical protein	-
spnnt_RS03135	665697..665984	+	288	WP_025172699.1	hypothetical protein	-
spnnt_RS03140	666053..666529	+	477	WP_016569768.1	helix-turn-helix domain-containing protein	Antitoxin
spnnt_RS03145	666529..667311	+	783	WP_016569769.1	zeta toxin family protein	Toxin
spnnt_RS03150	667508..668071	+	564	WP_016569771.1	hypothetical protein	-
spnnt_RS03155	668058..668291	+	234	WP_016397605.1	hypothetical protein	-
spnnt_RS03160	668281..669999	+	1719	WP_050074895.1	hypothetical protein	-
spnnt_RS03165	670333..670695	+	363	WP_016569773.1	hypothetical protein	-
spnnt_RS03170	670698..671063	+	366	WP_016569774.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	661709..662965	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 261 a.a.
• Molecular weight: 29752.62 Da
• Isoelectric Point: 5.6739

>T46682 WP_016569769.1 NZ_CP007593:666529-667311 [Streptococcus pneumoniae]
MALEAFRQDDFDLALKRTIRSLTRGKTVSTSPQAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSLHPNYLVLQE
EYGKDSVDYTKFFAGKMVEHLVDELSKDGYHLLIEGTLRTTEVPRKTAQLLKSRDYQVSLALIATKPELSYLSTLIRYEE
VYAENPSQARATSKEHHDGIVEHLVDNLRELETDKLFDQIQIYQRDKRCVYDSDTDDGSVADVLQDCLFGKWSKVEEEMI
KEAKEYLKRLSKSNNENILV

Nucleotide

• Length: 783 bp

>T46682 NZ_CP007593:666529-667311 [Streptococcus pneumoniae]
ATGGCATTAGAAGCATTTCGTCAAGATGACTTTGACCTAGCCCTGAAACGCACCATCCGTTCTTTAACCCGAGGCAAGAC
GGTTTCGACTAGCCCACAAGCTATTTTACTTGGGGGACAAAGCGGAGCAGGTAAGACGACCATACACCGGATTAAACAAA
AGGAATTTCAAGGCAATATCATCATTATTGATGGCGATAGCTACCGCTCTTTACACCCGAATTATCTTGTCTTACAAGAA
GAATATGGTAAAGATAGTGTGGATTACACCAAATTCTTTGCAGGAAAAATGGTAGAGCACCTCGTTGATGAACTCAGCAA
AGACGGTTATCATTTGTTAATTGAAGGAACCTTGAGAACGACAGAGGTTCCAAGAAAAACAGCTCAGCTATTGAAATCAA
GAGATTATCAAGTATCACTCGCATTGATTGCGACTAAGCCTGAATTGTCCTATCTCAGCACCTTAATTCGCTACGAGGAA
GTCTATGCCGAGAATCCAAGCCAAGCTAGAGCAACTTCTAAAGAGCATCACGATGGTATTGTAGAACATTTGGTTGATAA
TCTAAGAGAGTTAGAAACTGATAAACTCTTTGATCAGATTCAAATTTATCAACGAGACAAACGCTGTGTCTATGATTCTG
ATACAGACGATGGTTCAGTTGCTGATGTGTTGCAAGATTGTCTCTTCGGAAAATGGAGTAAGGTTGAGGAGGAGATGATT
AAGGAAGCTAAAGAATATTTAAAACGATTGAGTAAAAGTAATAATGAAAATATCTTAGTATGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18172.53 Da
• Isoelectric Point: 4.3936

>AT46682 WP_016569768.1 NZ_CP007593:666053-666529 [Streptococcus pneumoniae]
MIGENIKSLRRTHDLTQPEFAKIVGISRNSLSRYENGTSSVSTELIDRICQKFNVSYVDIVGEEKMLTPVEDYQLTLKIE
VIKERGAAVLSQLYRYQDSQGIAFDDETNPWILMSDDLSDLINTKIYLVDTFDEVERYNGYLDGIERMLELARHQVVA

Nucleotide

• Length: 477 bp

>AT46682 NZ_CP007593:666053-666529 [Streptococcus pneumoniae]
ATGATTGGAGAGAATATCAAATCATTACGCAGAACACACGATTTGACACAACCAGAGTTTGCCAAAATTGTTGGTATTTC
AAGAAATAGCTTGAGCCGTTACGAAAATGGGACAAGCTCTGTTTCGACAGAACTCATTGATCGAATTTGCCAAAAATTCA
ACGTGTCATATGTCGATATTGTAGGAGAAGAAAAGATGTTAACACCAGTAGAAGATTATCAATTGACGCTGAAAATAGAA
GTCATCAAAGAGCGTGGAGCAGCGGTTTTATCTCAACTGTATCGCTATCAAGATAGTCAAGGGATTGCTTTTGATGATGA
GACTAATCCTTGGATTTTGATGAGTGATGACTTGTCTGACCTTATCAATACCAAGATTTATCTGGTCGATACCTTTGATG
AGGTAGAACGCTACAATGGTTATTTGGATGGCATTGAGCGTATGTTGGAACTTGCTCGTCATCAGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A064C2H2