Detailed information of TA system TA046679

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4950964..4951799
• Replicon: chromosome
• Accession: NZ_CP007592
• Organism: Escherichia coli O157:H16 strain Santai

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1C0Y405
• Locus tag: CH99_RS24205
• Protein ID: WP_001774607.1
• Coordinates: 4950964..4951341 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M2U6P5
• Locus tag: CH99_RS24210
• Protein ID: WP_001774606.1
• Coordinates: 4951431..4951799 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH99_RS24180	4947055..4948593	-	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-
CH99_RS24190	4949343..4950185	-	843	WP_040235333.1	DUF4942 domain-containing protein	-
CH99_RS24195	4950270..4950467	-	198	WP_032152719.1	DUF957 domain-containing protein	-
CH99_RS24200	4950479..4950967	-	489	WP_001774608.1	hypothetical protein	-
CH99_RS24205	4950964..4951341	-	378	WP_001774607.1	TA system toxin CbtA family protein	Toxin
CH99_RS24210	4951431..4951799	-	369	WP_001774606.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CH99_RS24215	4951849..4952493	-	645	WP_001774605.1	hypothetical protein	-
CH99_RS24220	4952512..4952733	-	222	WP_000692312.1	DUF987 domain-containing protein	-
CH99_RS24225	4952802..4953278	-	477	WP_001297237.1	RadC family protein	-
CH99_RS24230	4953294..4953779	-	486	WP_040235172.1	antirestriction protein	-
CH99_RS24235	4953871..4954689	-	819	WP_040235173.1	DUF945 domain-containing protein	-
CH99_RS24240	4954779..4955012	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14218.20 Da
• Isoelectric Point: 7.8045

>T46679 WP_001774607.1 NZ_CP007592:c4951341-4950964 [Escherichia coli O157:H16]
MKTLPDTHVREASRCPPPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDTVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T46679 NZ_CP007592:c4951341-4950964 [Escherichia coli O157:H16]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATACGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.31 Da
• Isoelectric Point: 5.8746

>AT46679 WP_001774606.1 NZ_CP007592:c4951799-4951431 [Escherichia coli O157:H16]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT46679 NZ_CP007592:c4951799-4951431 [Escherichia coli O157:H16]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1C0Y405

Antitoxin

• AlphaFold DB: A0A1M2U6P5