Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4939242..4939739 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag CH99_RS24155 Protein ID WP_001672275.1
Coordinates 4939566..4939739 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CH99_RS24150 Protein ID WP_000398619.1
Coordinates 4939242..4939538 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS24130 4935973..4936692 - 720 WP_000611288.1 two-component system response regulator DcuR -
CH99_RS24135 4936689..4938320 - 1632 WP_001216477.1 sensor histidine kinase -
CH99_RS24140 4938501..4938731 + 231 WP_021557455.1 (4Fe-4S)-binding protein -
CH99_RS24145 4938743..4939015 + 273 WP_000405647.1 N-acetyltransferase -
CH99_RS24150 4939242..4939538 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CH99_RS24155 4939566..4939739 + 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
CH99_RS24160 4939858..4941375 - 1518 WP_001295074.1 lysine--tRNA ligase -
CH99_RS24165 4941612..4943069 - 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T46678 WP_001672275.1 NZ_CP007592:4939566-4939739 [Escherichia coli O157:H16]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T46678 NZ_CP007592:4939566-4939739 [Escherichia coli O157:H16]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT46678 WP_000398619.1 NZ_CP007592:4939242-4939538 [Escherichia coli O157:H16]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT46678 NZ_CP007592:4939242-4939538 [Escherichia coli O157:H16]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References