Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4155513..4156144 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name hicA Uniprot ID A0A1M1UJK7
Locus tag CH99_RS20540 Protein ID WP_001259382.1
Coordinates 4155869..4156144 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CH99_RS20535 Protein ID WP_000593555.1
Coordinates 4155513..4155872 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS20505 4150625..4151098 + 474 WP_001161650.1 PTS sugar transporter subunit IIA -
CH99_RS20510 4151095..4151376 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
CH99_RS20515 4151453..4152811 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
CH99_RS20520 4152804..4154312 + 1509 WP_021557288.1 xylulose kinase -
CH99_RS20525 4154302..4154571 + 270 WP_000933060.1 HPr family phosphocarrier protein -
CH99_RS20530 4154603..4155463 + 861 WP_021557289.1 class II aldolase -
CH99_RS20535 4155513..4155872 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CH99_RS20540 4155869..4156144 - 276 WP_001259382.1 type II toxin-antitoxin system HicA family toxin Toxin
CH99_RS20545 4156217..4157341 - 1125 WP_021557290.1 ABC transporter permease -
CH99_RS20550 4157341..4160076 - 2736 WP_000149130.1 ribosome-associated ATPase/putative transporter RbbA -
CH99_RS20555 4160073..4161140 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4149854..4165000 15146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.91 Da        Isoelectric Point: 10.7076

>T46676 WP_001259382.1 NZ_CP007592:c4156144-4155869 [Escherichia coli O157:H16]
MRTKVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T46676 NZ_CP007592:c4156144-4155869 [Escherichia coli O157:H16]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT46676 WP_000593555.1 NZ_CP007592:c4155872-4155513 [Escherichia coli O157:H16]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT46676 NZ_CP007592:c4155872-4155513 [Escherichia coli O157:H16]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1UJK7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References