Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3411223..3412064 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag CH99_RS16745 Protein ID WP_000854822.1
Coordinates 3411681..3412064 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M0CWL2
Locus tag CH99_RS16740 Protein ID WP_053271974.1
Coordinates 3411223..3411591 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS16720 3408968..3409786 + 819 WP_001234702.1 DUF945 domain-containing protein -
CH99_RS16725 3409877..3410362 + 486 WP_000214317.1 antirestriction protein -
CH99_RS16730 3410377..3410853 + 477 WP_001186747.1 RadC family protein -
CH99_RS16735 3410922..3411143 + 222 WP_000692312.1 DUF987 domain-containing protein -
CH99_RS16740 3411223..3411591 + 369 WP_053271974.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CH99_RS16745 3411681..3412064 + 384 WP_000854822.1 type IV toxin-antitoxin system toxin CbtA Toxin
CH99_RS16750 3412055..3412543 + 489 WP_001177592.1 hypothetical protein -
CH99_RS16755 3412555..3412752 + 198 WP_000839263.1 DUF957 domain-containing protein -
CH99_RS16760 3412849..3413694 + 846 WP_040234917.1 DUF4942 domain-containing protein -
CH99_RS16770 3413993..3414499 + 507 WP_040234919.1 G/U mismatch-specific DNA glycosylase -
CH99_RS16775 3414578..3416419 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T46672 WP_000854822.1 NZ_CP007592:3411681-3412064 [Escherichia coli O157:H16]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T46672 NZ_CP007592:3411681-3412064 [Escherichia coli O157:H16]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13679.41 Da        Isoelectric Point: 6.4758

>AT46672 WP_053271974.1 NZ_CP007592:3411223-3411591 [Escherichia coli O157:H16]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT46672 NZ_CP007592:3411223-3411591 [Escherichia coli O157:H16]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CWL2

References