Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3258319..3258902 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag CH99_RS15955 Protein ID WP_000254745.1
Coordinates 3258319..3258654 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2U551
Locus tag CH99_RS15960 Protein ID WP_021557150.1
Coordinates 3258654..3258902 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS15940 3254206..3255504 - 1299 WP_000036723.1 phosphopyruvate hydratase -
CH99_RS15945 3255592..3257229 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CH99_RS15950 3257457..3258248 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
CH99_RS15955 3258319..3258654 - 336 WP_000254745.1 endoribonuclease MazF Toxin
CH99_RS15960 3258654..3258902 - 249 WP_021557150.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CH99_RS15965 3258980..3261214 - 2235 WP_000226815.1 GTP pyrophosphokinase -
CH99_RS15970 3261262..3262563 - 1302 WP_021557151.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T46671 WP_000254745.1 NZ_CP007592:c3258654-3258319 [Escherichia coli O157:H16]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T46671 NZ_CP007592:c3258654-3258319 [Escherichia coli O157:H16]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9383.79 Da        Isoelectric Point: 4.4665

>AT46671 WP_021557150.1 NZ_CP007592:c3258902-3258654 [Escherichia coli O157:H16]
MIHSSVKRWGNSPAVRIPVTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT46671 NZ_CP007592:c3258902-3258654 [Escherichia coli O157:H16]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGTTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2U551

References