Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 841302..841920 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CH99_RS04185 Protein ID WP_001291435.1
Coordinates 841302..841520 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CH99_RS04190 Protein ID WP_000344800.1
Coordinates 841546..841920 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS04155 836592..837164 + 573 WP_000779826.1 lipoprotein -
CH99_RS04160 837195..837506 - 312 WP_000409911.1 MGMT family protein -
CH99_RS04165 837885..838238 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CH99_RS04170 838280..839830 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CH99_RS04175 839994..840464 - 471 WP_000136192.1 YlaC family protein -
CH99_RS04180 840580..841131 - 552 WP_000102564.1 maltose O-acetyltransferase -
CH99_RS04185 841302..841520 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CH99_RS04190 841546..841920 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CH99_RS04195 842466..845615 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CH99_RS04200 845638..846831 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T46660 WP_001291435.1 NZ_CP007592:c841520-841302 [Escherichia coli O157:H16]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T46660 NZ_CP007592:c841520-841302 [Escherichia coli O157:H16]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT46660 WP_000344800.1 NZ_CP007592:c841920-841546 [Escherichia coli O157:H16]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT46660 NZ_CP007592:c841920-841546 [Escherichia coli O157:H16]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References