Detailed information of TA system TA046658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 299804..300354
• Replicon: chromosome
• Accession: NZ_CP007592
• Organism: Escherichia coli O157:H16 strain Santai

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: CH99_RS01495
• Protein ID: WP_001160975.1
• Coordinates: 300040..300354 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: CH99_RS01490
• Protein ID: WP_000125565.1
• Coordinates: 299804..300037 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH99_RS01465	294875..295162	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
CH99_RS01470	295220..296551	+	1332	WP_001183198.1	MFS transporter	-
CH99_RS01475	296662..297192	+	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CH99_RS01480	297185..299047	+	1863	WP_040234481.1	glutathione-regulated potassium-efflux system protein KefC	-
CH99_RS01485	299239..299718	+	480	WP_001487721.1	type 3 dihydrofolate reductase	-
CH99_RS01490	299804..300037	+	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CH99_RS01495	300040..300354	+	315	WP_001160975.1	CcdB family protein	Toxin
CH99_RS01500	300351..301199	-	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CH99_RS01505	301206..301583	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CH99_RS01510	301586..302407	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CH99_RS01515	302404..303393	-	990	WP_021556398.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CH99_RS01520	303393..304679	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T46658 WP_001160975.1 NZ_CP007592:300040-300354 [Escherichia coli O157:H16]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T46658 NZ_CP007592:300040-300354 [Escherichia coli O157:H16]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT46658 WP_000125565.1 NZ_CP007592:299804-300037 [Escherichia coli O157:H16]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT46658 NZ_CP007592:299804-300037 [Escherichia coli O157:H16]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7