Detailed information of TA system TA046648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 200156..200764
• Replicon: chromosome
• Accession: NZ_CP007572
• Organism: Streptococcus agalactiae strain GBS6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I0S7M8
• Locus tag: DX05_RS01205
• Protein ID: WP_003038349.1
• Coordinates: 200156..200347 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DX05_RS01210
• Protein ID: WP_041326065.1
• Coordinates: 200384..200764 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DX05_RS01180	197391..197792	+	402	Protein_181	hypothetical protein	-
DX05_RS01185	197973..198416	+	444	WP_024053143.1	DUF1492 domain-containing protein	-
DX05_RS01190	198464..199042	+	579	WP_024053142.1	hypothetical protein	-
DX05_RS01195	199042..199377	+	336	WP_041326062.1	hypothetical protein	-
DX05_RS01205	200156..200347	+	192	WP_003038349.1	type II toxin-antitoxin system HicA family toxin	Toxin
DX05_RS01210	200384..200764	+	381	WP_041326065.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DX05_RS01215	201029..202288	-	1260	WP_041326067.1	tyrosine--tRNA ligase	-
DX05_RS01220	202399..204696	+	2298	WP_041326069.1	penicillin-binding protein PBP1B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	192991..269371	76380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-108)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7192.29 Da
• Isoelectric Point: 8.6749

>T46648 WP_003038349.1 NZ_CP007572:200156-200347 [Streptococcus agalactiae]
MPLTGKEMAKLAEKNGWVEIRQNGSHHHFKHEDFDYIVTIPIHGNKDLGRGLESKILKDLGLK

Nucleotide

• Length: 192 bp

>T46648 NZ_CP007572:200156-200347 [Streptococcus agalactiae]
ATGCCACTTACAGGGAAAGAAATGGCAAAGCTAGCCGAGAAAAACGGTTGGGTAGAAATCCGACAGAACGGCAGCCATCA
CCATTTCAAACATGAAGATTTTGATTATATAGTGACTATCCCAATCCATGGAAACAAGGATTTAGGAAGAGGGCTAGAAA
GCAAAATCCTGAAAGATTTGGGGCTGAAATAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14164.17 Da
• Isoelectric Point: 4.5235

>AT46648 WP_041326065.1 NZ_CP007572:200384-200764 [Streptococcus agalactiae]
MLKSYPAIFHKEEDGSYWVEFPEFGGGTQGANVEEALKNAREMLESVLAAYIDEGLDMPKPSDIMALTVKDGFVSMIQAD
PTPFIRNNKAIRKNVTVPEWLVRLADREKVNYSEVLTQALETKLQA

Nucleotide

• Length: 381 bp

>AT46648 NZ_CP007572:200384-200764 [Streptococcus agalactiae]
ATGTTAAAATCATATCCAGCAATTTTTCATAAAGAGGAAGACGGTTCTTATTGGGTAGAATTTCCAGAGTTTGGAGGAGG
CACACAAGGGGCAAATGTTGAAGAAGCCTTGAAAAATGCCCGTGAAATGCTTGAGAGTGTTTTAGCTGCTTACATAGACG
AAGGATTAGATATGCCAAAGCCTAGCGACATCATGGCTTTAACCGTTAAAGACGGCTTTGTTAGCATGATACAAGCAGAC
CCTACCCCGTTTATTCGCAACAATAAAGCCATTAGAAAGAATGTGACTGTCCCTGAATGGTTAGTAAGATTAGCGGATCG
TGAAAAAGTAAATTATTCTGAAGTGCTAACTCAAGCCCTAGAAACAAAATTACAAGCCTAG

Structures

Toxin

• AlphaFold DB: I0S7M8