Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2471053..2472767 Replicon chromosome
Accession NZ_CP007567
Organism Aeromonas media WS

Toxin (Protein)


Gene name pmenT Uniprot ID A0A6I4WWK8
Locus tag B224_RS11115 Protein ID WP_005331300.1
Coordinates 2472114..2472767 (-) Length 218 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A6I4WU85
Locus tag B224_RS11110 Protein ID WP_041206015.1
Coordinates 2471053..2472117 (-) Length 355 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B224_RS11100 2467134..2467847 - 714 WP_005331303.1 M48 family metallopeptidase -
B224_RS11105 2467847..2470978 - 3132 WP_041206014.1 HsdR family type I site-specific deoxyribonuclease -
B224_RS11110 2471053..2472117 - 1065 WP_041206015.1 macro domain-containing protein Antitoxin
B224_RS11115 2472114..2472767 - 654 WP_005331300.1 DUF4433 domain-containing protein Toxin
B224_RS23400 2473034..2473177 - 144 WP_005331298.1 hypothetical protein -
B224_RS11120 2473182..2474066 - 885 Protein_2195 IS30 family transposase -
B224_RS11125 2474167..2475435 + 1269 WP_041206016.1 IS4-like element ISAeme4 family transposase -
B224_RS11130 2475694..2476911 - 1218 WP_017411290.1 tetracycline efflux MFS transporter Tet(E) -
B224_RS11135 2476992..2477624 + 633 WP_017411289.1 tetracycline resistance transcriptional repressor TetR(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element tet(E) - 2436742..2502167 65425
- inside IScluster/Tn tet(E) - 2473182..2506795 33613


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-216)

Antitoxin

Macro domain

(18-131)


Sequences

Toxin        


Download         Length: 218 a.a.        Molecular weight: 25275.87 Da        Isoelectric Point: 7.0173

>T46642 WP_005331300.1 NZ_CP007567:c2472767-2472114 [Aeromonas media WS]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQSPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMNRVGVARPVYVRDGWYFR

Download         Length: 654 bp

>T46642 NZ_CP007567:c2472767-2472114 [Aeromonas media WS]
ATGGCCTATGACTATAGCCAGAGCCTGAATCCGCAGAAGGCGCTGATATTCCGGATCGTGCATCGGGATAACCTGGAATG
GATCCTGACCCATGGCCTGTGGGCGGGGAATGGTCAGCAGCAAAGCCCGCACTGGGTGCATATCGGGTTGCCGGAGCTGA
TTGCCAAGCGCGCGCGCCATCCGGTGCCGCTGGCTCCTTACGGCGTGTTGAATGACTATGTGCCCTTCTACTTCACGCCG
TTTTCTCCCATGCTCAGCAATATTCACTCCGGGCGCGGTGTTCCCCGGCGTGAAAATGATGAAATCCTGATCCTGGTTTC
CAGCCTGCATCGCATGGCTGAACTGGGGCTACCGTTTGTGTTTACCGATAGCCATGCCTATTACCAGTGGGCCAATTTCT
ATGTCGACCTTGGCGATCTGGGGCGGATTGACTGGCCACTGTTGCAGCGCCGTGATTTCAAGCGTGATGCGGACGATTTG
AGCAAGCTGGAACGCTATCAGGCCGAGGCCTTGATCCATCAACATTGCCCCATCGAGGCGTTGCTGGGCGTGGTGTGTTA
CAACGAAGAAATAAAGAACAGCGTGGAACAATGCATGAATCGGGTGGGGGTCGCGCGCCCCGTCTATGTCCGTGATGGGT
GGTATTTCAGATGA

Antitoxin


Download         Length: 355 a.a.        Molecular weight: 39588.70 Da        Isoelectric Point: 6.5002

>AT46642 WP_041206015.1 NZ_CP007567:c2472117-2471053 [Aeromonas media WS]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Download         Length: 1065 bp

>AT46642 NZ_CP007567:c2472117-2471053 [Aeromonas media WS]
ATGATTCGATATACAACAGGCAATCTGCTGGATGCTCCGGTGGAAGCGCTGGTGAATACCGTCAACACCGTCGGTGTCAT
GGGCAAGGGGATCGCCTTGATGTTCAAGGAGCGGTTCCCGGCCAATATGCGCGCCTATGCCGAGGCGTGCAAACAGGGTG
ATGTCGTCACCGGCAAGATGTTTATTACCCAGACGGGTGAGCTGCTGGGGCCGCGCTGGATCGTGAATTTTCCGACCAAA
CAGCACTGGCGCAATCCGGCACAGATGGCGTGGATCCAGGATGGTTTGCAGGATCTACGGGCCTGGTTACAGGCGCAGCA
GGTCTCTTCGATTGCCATTCCGCCGCTGGGGGCTGGCAACGGTGGTCTGCCTTGGCCAGAGGTCAAACAGGCGATTGAAA
CGGCACTGGGCGATCTGGATATCGATATCCTGCTCTATGAGCCCAGTAGCCAATACCAAAATGTGGCCAAGCGGGGCGGG
GTGGCGGAGCTGACTCCGGCTCGGGCCTTGATTGCTGAACTGGTACGCCGCTACTGGTTTTTAGGAATGGAGTGCAGCTT
GCTGGAGATCCAGAAACTGGCCTGGTTTCTGGCTCGGGTCATTGGCCGTAACCCGCTGGGGGATCCGCTGCAGTTGCAGT
TTAAGGCCCATCGTTATGGCCCTTATGCCAACAATCTGACCCATCTGCTCAATGCGATGGACGGCAGTTATCTGCAGTCC
GACAAGCGCATTCCGGATAGCAAGCCGTTTGATGTCATCTGGTTTAATGAGGCCAAAAAACAAGAGCTTGCCGTTTATCT
CCAGACCGAGGCCAAAGCATTTTTACCGATACTGGAGCAGACGACCGCGTTAATCGATGGTTTTGAGTCGCCCTATGGCA
TGGAGTTATTGGCCACGGTCGATTGGTTGATCGCCGAAGAGGGCTGTGCGCCGACGCTGAGCGCGCTGCGAGAGGGGTTG
CATCGCTGGCCAGCGGGAACCCCGTGGGGTGAGCGCAAGGCGCGGCTGTTTGATGATCGCAGTCTGCAGATCGCCTTGAC
CCGTTTGCAGCAGGGGTTGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I4WWK8

Antitoxin

Source ID Structure
AlphaFold DB A0A6I4WU85

References